Experiment: 36hr PHYTO10 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS33475 and BPHYT_RS33480 are separated by 307 nucleotides BPHYT_RS33480 and BPHYT_RS33485 are separated by 289 nucleotides BPHYT_RS33485 and BPHYT_RS33490 are separated by 122 nucleotides
BPHYT_RS33475: BPHYT_RS33475 - threonine transporter, at 3,096,497 to 3,097,132
_RS33475
BPHYT_RS33480: BPHYT_RS33480 - cold-shock protein, at 3,097,440 to 3,097,643
_RS33480
BPHYT_RS33485: BPHYT_RS33485 - hypothetical protein, at 3,097,933 to 3,098,157
_RS33485
BPHYT_RS33490: BPHYT_RS33490 - acyl-CoA synthetase, at 3,098,280 to 3,099,911
_RS33490
Position (kb)
3097
3098
3099 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3096.941 kb on + strand, within BPHYT_RS33475 at 3096.941 kb on + strand, within BPHYT_RS33475 at 3096.941 kb on + strand, within BPHYT_RS33475 at 3096.941 kb on + strand, within BPHYT_RS33475 at 3096.941 kb on + strand, within BPHYT_RS33475 at 3096.942 kb on - strand, within BPHYT_RS33475 at 3096.986 kb on + strand, within BPHYT_RS33475 at 3096.987 kb on - strand, within BPHYT_RS33475 at 3096.987 kb on - strand, within BPHYT_RS33475 at 3097.034 kb on + strand, within BPHYT_RS33475 at 3097.263 kb on + strand at 3097.264 kb on - strand at 3097.927 kb on + strand at 3097.947 kb on + strand at 3097.948 kb on - strand at 3097.948 kb on - strand at 3097.948 kb on - strand at 3097.948 kb on - strand at 3097.957 kb on + strand, within BPHYT_RS33485 at 3097.957 kb on + strand, within BPHYT_RS33485 at 3097.958 kb on - strand, within BPHYT_RS33485 at 3097.967 kb on - strand, within BPHYT_RS33485 at 3097.967 kb on - strand, within BPHYT_RS33485 at 3098.072 kb on - strand, within BPHYT_RS33485 at 3098.235 kb on + strand at 3098.236 kb on - strand at 3098.236 kb on - strand at 3098.271 kb on - strand at 3098.387 kb on - strand at 3098.514 kb on + strand, within BPHYT_RS33490 at 3098.515 kb on - strand, within BPHYT_RS33490 at 3098.515 kb on - strand, within BPHYT_RS33490 at 3098.536 kb on + strand, within BPHYT_RS33490 at 3098.536 kb on + strand, within BPHYT_RS33490 at 3098.707 kb on + strand, within BPHYT_RS33490 at 3098.821 kb on + strand, within BPHYT_RS33490 at 3098.915 kb on - strand, within BPHYT_RS33490
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 10 remove 3,096,941 + BPHYT_RS33475 0.70 +1.0 3,096,941 + BPHYT_RS33475 0.70 +0.1 3,096,941 + BPHYT_RS33475 0.70 +0.8 3,096,941 + BPHYT_RS33475 0.70 -2.4 3,096,941 + BPHYT_RS33475 0.70 -0.3 3,096,942 - BPHYT_RS33475 0.70 +0.3 3,096,986 + BPHYT_RS33475 0.77 -1.3 3,096,987 - BPHYT_RS33475 0.77 -0.3 3,096,987 - BPHYT_RS33475 0.77 +1.5 3,097,034 + BPHYT_RS33475 0.84 -0.7 3,097,263 + +0.1 3,097,264 - -0.1 3,097,927 + -0.1 3,097,947 + +0.3 3,097,948 - -0.4 3,097,948 - -0.5 3,097,948 - -0.5 3,097,948 - +0.5 3,097,957 + BPHYT_RS33485 0.11 +0.5 3,097,957 + BPHYT_RS33485 0.11 +0.9 3,097,958 - BPHYT_RS33485 0.11 +0.5 3,097,967 - BPHYT_RS33485 0.15 -0.8 3,097,967 - BPHYT_RS33485 0.15 -0.3 3,098,072 - BPHYT_RS33485 0.62 +0.5 3,098,235 + +0.2 3,098,236 - -0.1 3,098,236 - -3.1 3,098,271 - -0.2 3,098,387 - -0.0 3,098,514 + BPHYT_RS33490 0.14 +0.3 3,098,515 - BPHYT_RS33490 0.14 +0.5 3,098,515 - BPHYT_RS33490 0.14 -0.2 3,098,536 + BPHYT_RS33490 0.16 +0.0 3,098,536 + BPHYT_RS33490 0.16 -0.7 3,098,707 + BPHYT_RS33490 0.26 -0.1 3,098,821 + BPHYT_RS33490 0.33 +0.0 3,098,915 - BPHYT_RS33490 0.39 -2.6
Or see this region's nucleotide sequence