Experiment: 36hr PHYTO10 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS33455 and BPHYT_RS33460 are separated by 3 nucleotides BPHYT_RS33460 and BPHYT_RS33465 are separated by 147 nucleotides BPHYT_RS33465 and BPHYT_RS33470 are separated by 154 nucleotides BPHYT_RS33470 and BPHYT_RS33475 are separated by 465 nucleotides
BPHYT_RS33455: BPHYT_RS33455 - protocatechuate 3,4-dioxygenase subunit beta, at 3,092,938 to 3,093,642
_RS33455
BPHYT_RS33460: BPHYT_RS33460 - protocatechuate 3,4-dioxygenase subunit alpha, at 3,093,646 to 3,094,233
_RS33460
BPHYT_RS33465: BPHYT_RS33465 - D-alanyl-D-alanine carboxypeptidase, at 3,094,381 to 3,095,613
_RS33465
BPHYT_RS33470: BPHYT_RS33470 - DNA-binding protein, at 3,095,768 to 3,096,031
_RS33470
BPHYT_RS33475: BPHYT_RS33475 - threonine transporter, at 3,096,497 to 3,097,132
_RS33475
Position (kb)
3094
3095
3096 Strain fitness (log2 ratio)
-2
-1
0
1 at 3093.412 kb on - strand, within BPHYT_RS33455 at 3093.589 kb on + strand at 3093.688 kb on + strand at 3094.066 kb on + strand, within BPHYT_RS33460 at 3094.255 kb on + strand at 3094.269 kb on - strand at 3094.288 kb on + strand at 3094.570 kb on + strand, within BPHYT_RS33465 at 3094.765 kb on - strand, within BPHYT_RS33465 at 3094.875 kb on + strand, within BPHYT_RS33465 at 3094.876 kb on - strand, within BPHYT_RS33465 at 3095.159 kb on - strand, within BPHYT_RS33465 at 3095.269 kb on - strand, within BPHYT_RS33465 at 3095.770 kb on - strand at 3095.852 kb on - strand, within BPHYT_RS33470 at 3095.852 kb on - strand, within BPHYT_RS33470 at 3095.852 kb on - strand, within BPHYT_RS33470 at 3095.852 kb on - strand, within BPHYT_RS33470 at 3095.852 kb on - strand, within BPHYT_RS33470 at 3095.852 kb on - strand at 3095.852 kb on - strand, within BPHYT_RS33470 at 3095.854 kb on + strand, within BPHYT_RS33470 at 3095.854 kb on + strand, within BPHYT_RS33470 at 3095.872 kb on - strand, within BPHYT_RS33470 at 3095.932 kb on + strand, within BPHYT_RS33470 at 3096.036 kb on + strand at 3096.036 kb on + strand at 3096.037 kb on - strand at 3096.054 kb on + strand at 3096.054 kb on + strand at 3096.071 kb on - strand at 3096.103 kb on + strand at 3096.124 kb on - strand at 3096.124 kb on - strand at 3096.276 kb on - strand at 3096.291 kb on - strand at 3096.392 kb on - strand at 3096.453 kb on + strand at 3096.453 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 10 remove 3,093,412 - BPHYT_RS33455 0.67 -0.4 3,093,589 + -0.8 3,093,688 + +0.1 3,094,066 + BPHYT_RS33460 0.71 -0.2 3,094,255 + -2.0 3,094,269 - -1.3 3,094,288 + +1.1 3,094,570 + BPHYT_RS33465 0.15 -1.3 3,094,765 - BPHYT_RS33465 0.31 -0.5 3,094,875 + BPHYT_RS33465 0.40 -0.8 3,094,876 - BPHYT_RS33465 0.40 +0.7 3,095,159 - BPHYT_RS33465 0.63 -1.0 3,095,269 - BPHYT_RS33465 0.72 -1.0 3,095,770 - +1.5 3,095,852 - BPHYT_RS33470 0.32 -0.8 3,095,852 - BPHYT_RS33470 0.32 -0.2 3,095,852 - BPHYT_RS33470 0.32 -0.5 3,095,852 - BPHYT_RS33470 0.32 -1.9 3,095,852 - BPHYT_RS33470 0.32 -1.2 3,095,852 - -1.0 3,095,852 - BPHYT_RS33470 0.32 -0.0 3,095,854 + BPHYT_RS33470 0.33 -1.3 3,095,854 + BPHYT_RS33470 0.33 +0.0 3,095,872 - BPHYT_RS33470 0.39 +0.7 3,095,932 + BPHYT_RS33470 0.62 +0.7 3,096,036 + -0.8 3,096,036 + -0.8 3,096,037 - -0.5 3,096,054 + -0.9 3,096,054 + -0.0 3,096,071 - -1.0 3,096,103 + -0.7 3,096,124 - -0.1 3,096,124 - +0.2 3,096,276 - -0.1 3,096,291 - -0.1 3,096,392 - +0.4 3,096,453 + -0.0 3,096,453 + -0.8
Or see this region's nucleotide sequence