Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS31300

Experiment: 36hr PHYTO10 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS31295 and BPHYT_RS31300 are separated by 645 nucleotidesBPHYT_RS31300 and BPHYT_RS31305 are separated by 81 nucleotides BPHYT_RS31295: BPHYT_RS31295 - LuxR family transcriptional regulator, at 2,580,082 to 2,580,732 _RS31295 BPHYT_RS31300: BPHYT_RS31300 - Crp/Fnr family transcriptional regulator, at 2,581,378 to 2,582,166 _RS31300 BPHYT_RS31305: BPHYT_RS31305 - alcohol dehydrogenase, at 2,582,248 to 2,583,234 _RS31305 Position (kb) 2581 2582 2583Strain fitness (log2 ratio) -3 -2 -1 0 1at 2580.451 kb on + strand, within BPHYT_RS31295at 2580.451 kb on + strand, within BPHYT_RS31295at 2580.452 kb on - strand, within BPHYT_RS31295at 2580.452 kb on - strand, within BPHYT_RS31295at 2580.629 kb on - strand, within BPHYT_RS31295at 2580.629 kb on - strand, within BPHYT_RS31295at 2580.700 kb on - strandat 2580.815 kb on + strandat 2580.815 kb on + strandat 2580.933 kb on - strandat 2581.313 kb on + strandat 2581.519 kb on - strand, within BPHYT_RS31300at 2581.643 kb on - strand, within BPHYT_RS31300at 2581.765 kb on - strand, within BPHYT_RS31300at 2582.677 kb on - strand, within BPHYT_RS31305at 2582.732 kb on + strand, within BPHYT_RS31305

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 10
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2,580,451 + BPHYT_RS31295 0.57 -1.3
2,580,451 + BPHYT_RS31295 0.57 -1.5
2,580,452 - BPHYT_RS31295 0.57 -0.2
2,580,452 - BPHYT_RS31295 0.57 +0.0
2,580,629 - BPHYT_RS31295 0.84 +1.0
2,580,629 - BPHYT_RS31295 0.84 -0.2
2,580,700 - +1.1
2,580,815 + -0.8
2,580,815 + -1.0
2,580,933 - -0.5
2,581,313 + +0.1
2,581,519 - BPHYT_RS31300 0.18 -0.5
2,581,643 - BPHYT_RS31300 0.34 -1.2
2,581,765 - BPHYT_RS31300 0.49 -0.7
2,582,677 - BPHYT_RS31305 0.43 -3.3
2,582,732 + BPHYT_RS31305 0.49 +0.8

Or see this region's nucleotide sequence