Experiment: 36hr PHYTO10 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23985 and BPHYT_RS23990 are separated by 405 nucleotides BPHYT_RS23990 and BPHYT_RS23995 are separated by 12 nucleotides BPHYT_RS23995 and BPHYT_RS24000 are separated by 293 nucleotides
BPHYT_RS23985: BPHYT_RS23985 - carbohydrate porin, at 946,724 to 948,247
_RS23985
BPHYT_RS23990: BPHYT_RS23990 - N-hydroxyarylamine O-acetyltransferase, at 948,653 to 949,492
_RS23990
BPHYT_RS23995: BPHYT_RS23995 - isoprenylcysteine carboxyl methyltransferase, at 949,505 to 950,185
_RS23995
BPHYT_RS24000: BPHYT_RS24000 - L-histidine ABC transporter, substrate-binding component <small>(from data)</small>, at 950,479 to 951,339
_RS24000
Position (kb) 948
949
950 Strain fitness (log2 ratio) -2
-1
0
1
2 at 947.772 kb on - strand, within BPHYT_RS23985 at 947.772 kb on - strand, within BPHYT_RS23985 at 947.941 kb on - strand, within BPHYT_RS23985 at 948.361 kb on - strand at 948.424 kb on - strand at 948.446 kb on + strand at 948.446 kb on + strand at 948.467 kb on - strand at 948.483 kb on - strand at 948.676 kb on + strand at 949.185 kb on - strand, within BPHYT_RS23990 at 949.901 kb on + strand, within BPHYT_RS23995 at 949.901 kb on + strand, within BPHYT_RS23995 at 949.943 kb on - strand, within BPHYT_RS23995 at 949.989 kb on - strand, within BPHYT_RS23995 at 950.078 kb on - strand, within BPHYT_RS23995 at 950.141 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 10 remove 947,772 - BPHYT_RS23985 0.69 -1.8 947,772 - BPHYT_RS23985 0.69 +0.1 947,941 - BPHYT_RS23985 0.80 +1.2 948,361 - +0.6 948,424 - +1.0 948,446 + -0.2 948,446 + +0.1 948,467 - -1.2 948,483 - +0.2 948,676 + +2.1 949,185 - BPHYT_RS23990 0.63 -1.4 949,901 + BPHYT_RS23995 0.58 -0.1 949,901 + BPHYT_RS23995 0.58 +0.5 949,943 - BPHYT_RS23995 0.64 -0.5 949,989 - BPHYT_RS23995 0.71 +0.2 950,078 - BPHYT_RS23995 0.84 -0.2 950,141 + +2.5
Or see this region's nucleotide sequence