Experiment: 36hr PHYTO10 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23925 and BPHYT_RS23930 overlap by 13 nucleotides BPHYT_RS23930 and BPHYT_RS23935 are separated by 2 nucleotides BPHYT_RS23935 and BPHYT_RS23940 are separated by 8 nucleotides BPHYT_RS23940 and BPHYT_RS23945 are separated by 174 nucleotides
BPHYT_RS23925: BPHYT_RS23925 - malonate decarboxylase subunit delta, at 933,095 to 933,409
_RS23925
BPHYT_RS23930: BPHYT_RS23930 - triphosphoribosyl-dephospho-CoA synthase, at 933,397 to 934,320
_RS23930
BPHYT_RS23935: BPHYT_RS23935 - malonate decarboxylase subunit alpha, at 934,323 to 936,005
_RS23935
BPHYT_RS23940: BPHYT_RS23940 - GntR family transcriptional regulator, at 936,014 to 936,787
_RS23940
BPHYT_RS23945: BPHYT_RS23945 - MFS transporter, at 936,962 to 938,263
_RS23945
Position (kb) 934
935
936
937 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 934.128 kb on + strand, within BPHYT_RS23930 at 934.128 kb on + strand, within BPHYT_RS23930 at 934.355 kb on - strand at 934.355 kb on - strand at 934.503 kb on + strand, within BPHYT_RS23935 at 935.162 kb on - strand, within BPHYT_RS23935 at 935.223 kb on + strand, within BPHYT_RS23935 at 935.224 kb on - strand, within BPHYT_RS23935 at 935.486 kb on - strand, within BPHYT_RS23935 at 935.564 kb on - strand, within BPHYT_RS23935 at 935.575 kb on + strand, within BPHYT_RS23935 at 935.575 kb on + strand, within BPHYT_RS23935 at 935.575 kb on + strand, within BPHYT_RS23935 at 935.576 kb on - strand, within BPHYT_RS23935 at 935.579 kb on + strand, within BPHYT_RS23935 at 935.580 kb on - strand, within BPHYT_RS23935 at 935.581 kb on + strand, within BPHYT_RS23935 at 935.666 kb on + strand, within BPHYT_RS23935 at 936.012 kb on - strand at 936.099 kb on - strand, within BPHYT_RS23940 at 936.109 kb on - strand, within BPHYT_RS23940 at 936.916 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 10 remove 934,128 + BPHYT_RS23930 0.79 +2.0 934,128 + BPHYT_RS23930 0.79 -0.2 934,355 - +0.3 934,355 - -1.0 934,503 + BPHYT_RS23935 0.11 -0.1 935,162 - BPHYT_RS23935 0.50 +3.0 935,223 + BPHYT_RS23935 0.53 -1.2 935,224 - BPHYT_RS23935 0.54 -1.1 935,486 - BPHYT_RS23935 0.69 +0.4 935,564 - BPHYT_RS23935 0.74 +0.9 935,575 + BPHYT_RS23935 0.74 -0.4 935,575 + BPHYT_RS23935 0.74 +1.0 935,575 + BPHYT_RS23935 0.74 +2.8 935,576 - BPHYT_RS23935 0.74 -1.5 935,579 + BPHYT_RS23935 0.75 -0.9 935,580 - BPHYT_RS23935 0.75 -0.9 935,581 + BPHYT_RS23935 0.75 +1.0 935,666 + BPHYT_RS23935 0.80 +2.0 936,012 - -0.7 936,099 - BPHYT_RS23940 0.11 +1.9 936,109 - BPHYT_RS23940 0.12 -1.5 936,916 - -3.3
Or see this region's nucleotide sequence