Experiment: 36hr PHYTO10 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23475 and BPHYT_RS23480 are separated by 339 nucleotides BPHYT_RS23480 and BPHYT_RS23485 are separated by 27 nucleotides BPHYT_RS23485 and BPHYT_RS23490 are separated by 105 nucleotides BPHYT_RS23490 and BPHYT_RS23495 are separated by 214 nucleotides
BPHYT_RS23475: BPHYT_RS23475 - luciferase, at 834,026 to 835,033
_RS23475
BPHYT_RS23480: BPHYT_RS23480 - hypothetical protein, at 835,373 to 835,894
_RS23480
BPHYT_RS23485: BPHYT_RS23485 - glyoxalase, at 835,922 to 836,344
_RS23485
BPHYT_RS23490: BPHYT_RS23490 - hypothetical protein, at 836,450 to 836,752
_RS23490
BPHYT_RS23495: BPHYT_RS23495 - thioredoxin reductase, at 836,967 to 838,700
_RS23495
Position (kb) 835
836
837 Strain fitness (log2 ratio) -2
-1
0
1 at 835.028 kb on - strand at 835.172 kb on - strand at 835.280 kb on + strand at 835.326 kb on + strand at 835.327 kb on - strand at 835.535 kb on + strand, within BPHYT_RS23480 at 835.536 kb on - strand, within BPHYT_RS23480 at 835.917 kb on - strand at 836.742 kb on - strand at 837.279 kb on + strand, within BPHYT_RS23495
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 10 remove 835,028 - +0.4 835,172 - +0.9 835,280 + +0.1 835,326 + -2.5 835,327 - +0.6 835,535 + BPHYT_RS23480 0.31 -0.5 835,536 - BPHYT_RS23480 0.31 -0.7 835,917 - +0.8 836,742 - -1.1 837,279 + BPHYT_RS23495 0.18 -2.4
Or see this region's nucleotide sequence