Experiment: 36hr PHYTO10 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23300 and BPHYT_RS23305 are separated by 45 nucleotides BPHYT_RS23305 and BPHYT_RS23310 are separated by 145 nucleotides BPHYT_RS23310 and BPHYT_RS23315 are separated by 82 nucleotides BPHYT_RS23315 and BPHYT_RS23320 are separated by 123 nucleotides
BPHYT_RS23300: BPHYT_RS23300 - IclR family transcriptional regulator, at 790,401 to 791,012
_RS23300
BPHYT_RS23305: BPHYT_RS23305 - hypothetical protein, at 791,058 to 791,600
_RS23305
BPHYT_RS23310: BPHYT_RS23310 - hypothetical protein, at 791,746 to 792,558
_RS23310
BPHYT_RS23315: BPHYT_RS23315 - hypothetical protein, at 792,641 to 793,042
_RS23315
BPHYT_RS23320: BPHYT_RS23320 - hypothetical protein, at 793,166 to 793,630
_RS23320
Position (kb) 791
792
793 Strain fitness (log2 ratio) -2
-1
0
1
2 at 791.025 kb on + strand at 791.026 kb on - strand at 791.080 kb on + strand at 791.202 kb on - strand, within BPHYT_RS23305 at 791.314 kb on + strand, within BPHYT_RS23305 at 791.314 kb on + strand, within BPHYT_RS23305 at 791.496 kb on - strand, within BPHYT_RS23305 at 791.721 kb on + strand at 791.722 kb on - strand at 791.724 kb on - strand at 791.774 kb on + strand at 791.775 kb on - strand at 791.903 kb on - strand, within BPHYT_RS23310 at 792.082 kb on + strand, within BPHYT_RS23310 at 792.181 kb on + strand, within BPHYT_RS23310 at 792.218 kb on - strand, within BPHYT_RS23310 at 792.590 kb on + strand at 792.613 kb on + strand at 792.897 kb on - strand, within BPHYT_RS23315 at 793.137 kb on + strand at 793.335 kb on + strand, within BPHYT_RS23320 at 793.435 kb on + strand, within BPHYT_RS23320 at 793.470 kb on - strand, within BPHYT_RS23320 at 793.477 kb on - strand, within BPHYT_RS23320 at 793.481 kb on + strand, within BPHYT_RS23320
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 10 remove 791,025 + +0.2 791,026 - -0.4 791,080 + +0.1 791,202 - BPHYT_RS23305 0.27 -0.3 791,314 + BPHYT_RS23305 0.47 -0.2 791,314 + BPHYT_RS23305 0.47 +0.1 791,496 - BPHYT_RS23305 0.81 -0.1 791,721 + -1.2 791,722 - -0.3 791,724 - +0.1 791,774 + -1.3 791,775 - +0.9 791,903 - BPHYT_RS23310 0.19 +0.1 792,082 + BPHYT_RS23310 0.41 -0.9 792,181 + BPHYT_RS23310 0.54 -1.5 792,218 - BPHYT_RS23310 0.58 +0.1 792,590 + -0.1 792,613 + +1.8 792,897 - BPHYT_RS23315 0.64 -2.2 793,137 + -0.2 793,335 + BPHYT_RS23320 0.36 -0.3 793,435 + BPHYT_RS23320 0.58 +0.9 793,470 - BPHYT_RS23320 0.65 -0.8 793,477 - BPHYT_RS23320 0.67 +0.1 793,481 + BPHYT_RS23320 0.68 +0.4
Or see this region's nucleotide sequence