Experiment: 36hr PHYTO10 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS18620 and BPHYT_RS18625 are separated by 205 nucleotides BPHYT_RS18625 and BPHYT_RS18630 overlap by 4 nucleotides BPHYT_RS18630 and BPHYT_RS18635 are separated by 103 nucleotides
BPHYT_RS18620: BPHYT_RS18620 - transcriptional regulator, at 4,188,364 to 4,188,876
_RS18620
BPHYT_RS18625: BPHYT_RS18625 - allophanate hydrolase, at 4,189,082 to 4,189,735
_RS18625
BPHYT_RS18630: BPHYT_RS18630 - allophanate hydrolase, at 4,189,732 to 4,190,784
_RS18630
BPHYT_RS18635: BPHYT_RS18635 - LamB/YcsF family protein, at 4,190,888 to 4,191,640
_RS18635
Position (kb)
4189
4190
4191 Strain fitness (log2 ratio)
-2
-1
0
1 at 4188.921 kb on - strand at 4188.990 kb on - strand at 4188.995 kb on - strand at 4189.076 kb on + strand at 4189.093 kb on + strand at 4189.093 kb on + strand at 4189.094 kb on - strand at 4189.094 kb on - strand at 4189.122 kb on + strand at 4189.123 kb on - strand at 4189.454 kb on + strand, within BPHYT_RS18625 at 4189.455 kb on - strand, within BPHYT_RS18625 at 4189.455 kb on - strand, within BPHYT_RS18625 at 4189.493 kb on + strand, within BPHYT_RS18625 at 4189.493 kb on + strand, within BPHYT_RS18625 at 4189.494 kb on - strand, within BPHYT_RS18625 at 4189.523 kb on - strand, within BPHYT_RS18625 at 4189.523 kb on - strand, within BPHYT_RS18625 at 4189.726 kb on - strand at 4190.066 kb on - strand, within BPHYT_RS18630 at 4190.475 kb on + strand, within BPHYT_RS18630 at 4190.703 kb on + strand at 4190.704 kb on - strand at 4190.704 kb on - strand at 4190.707 kb on + strand at 4190.708 kb on - strand at 4190.708 kb on - strand at 4190.782 kb on + strand at 4190.783 kb on - strand at 4191.122 kb on + strand, within BPHYT_RS18635 at 4191.137 kb on + strand, within BPHYT_RS18635 at 4191.138 kb on - strand, within BPHYT_RS18635 at 4191.774 kb on - strand at 4191.774 kb on - strand at 4191.774 kb on - strand at 4191.774 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 10 remove 4,188,921 - -0.5 4,188,990 - +0.2 4,188,995 - -1.0 4,189,076 + -1.2 4,189,093 + -1.3 4,189,093 + -0.0 4,189,094 - +0.6 4,189,094 - -0.7 4,189,122 + +1.2 4,189,123 - -0.5 4,189,454 + BPHYT_RS18625 0.57 -0.9 4,189,455 - BPHYT_RS18625 0.57 -0.3 4,189,455 - BPHYT_RS18625 0.57 -0.3 4,189,493 + BPHYT_RS18625 0.63 -0.9 4,189,493 + BPHYT_RS18625 0.63 +1.1 4,189,494 - BPHYT_RS18625 0.63 -0.6 4,189,523 - BPHYT_RS18625 0.67 +1.5 4,189,523 - BPHYT_RS18625 0.67 +0.4 4,189,726 - -0.5 4,190,066 - BPHYT_RS18630 0.32 -0.7 4,190,475 + BPHYT_RS18630 0.71 -2.4 4,190,703 + +0.9 4,190,704 - -0.4 4,190,704 - +0.5 4,190,707 + +0.4 4,190,708 - -0.2 4,190,708 - -0.0 4,190,782 + +0.2 4,190,783 - -0.4 4,191,122 + BPHYT_RS18635 0.31 +0.6 4,191,137 + BPHYT_RS18635 0.33 +1.5 4,191,138 - BPHYT_RS18635 0.33 +0.5 4,191,774 - +0.1 4,191,774 - -0.4 4,191,774 - +1.1 4,191,774 - -0.6
Or see this region's nucleotide sequence