Experiment: 36hr PHYTO10 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS17400 and BPHYT_RS17405 are separated by 30 nucleotides BPHYT_RS17405 and BPHYT_RS17410 are separated by 46 nucleotides BPHYT_RS17410 and BPHYT_RS17415 are separated by 84 nucleotides
BPHYT_RS17400: BPHYT_RS17400 - anthranilate phosphoribosyltransferase, at 3,942,611 to 3,943,642
_RS17400
BPHYT_RS17405: BPHYT_RS17405 - indole-3-glycerol phosphate synthase, at 3,943,673 to 3,944,458
_RS17405
BPHYT_RS17410: BPHYT_RS17410 - adenylate cyclase, at 3,944,505 to 3,945,131
_RS17410
BPHYT_RS17415: BPHYT_RS17415 - uracil-DNA glycosylase, at 3,945,216 to 3,946,061
_RS17415
Position (kb)
3944
3945
3946 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3943.517 kb on + strand, within BPHYT_RS17400 at 3943.518 kb on - strand, within BPHYT_RS17400 at 3943.518 kb on - strand, within BPHYT_RS17400 at 3943.648 kb on + strand at 3943.661 kb on - strand at 3943.672 kb on + strand at 3943.672 kb on + strand at 3943.672 kb on + strand at 3943.672 kb on + strand at 3943.672 kb on + strand at 3943.673 kb on - strand at 3943.673 kb on - strand at 3943.673 kb on - strand at 3943.673 kb on - strand at 3943.673 kb on - strand at 3943.694 kb on - strand at 3943.985 kb on + strand, within BPHYT_RS17405 at 3944.492 kb on - strand at 3944.492 kb on - strand at 3944.618 kb on + strand, within BPHYT_RS17410 at 3944.622 kb on + strand, within BPHYT_RS17410 at 3944.623 kb on - strand, within BPHYT_RS17410 at 3945.067 kb on + strand, within BPHYT_RS17410 at 3945.067 kb on + strand, within BPHYT_RS17410 at 3945.112 kb on - strand at 3946.059 kb on + strand at 3946.059 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 10 remove 3,943,517 + BPHYT_RS17400 0.88 -0.2 3,943,518 - BPHYT_RS17400 0.88 -1.7 3,943,518 - BPHYT_RS17400 0.88 +0.4 3,943,648 + +0.0 3,943,661 - -0.2 3,943,672 + +0.2 3,943,672 + -0.3 3,943,672 + +1.4 3,943,672 + -0.7 3,943,672 + -0.7 3,943,673 - +0.6 3,943,673 - +0.2 3,943,673 - -0.3 3,943,673 - -0.4 3,943,673 - +0.6 3,943,694 - +0.1 3,943,985 + BPHYT_RS17405 0.40 -0.1 3,944,492 - -1.0 3,944,492 - +0.2 3,944,618 + BPHYT_RS17410 0.18 +0.7 3,944,622 + BPHYT_RS17410 0.19 +3.4 3,944,623 - BPHYT_RS17410 0.19 +0.7 3,945,067 + BPHYT_RS17410 0.90 +0.0 3,945,067 + BPHYT_RS17410 0.90 +0.1 3,945,112 - +0.6 3,946,059 + -0.7 3,946,059 + +0.1
Or see this region's nucleotide sequence