Experiment: 36hr PHYTO10 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS16980 and BPHYT_RS16985 are separated by 206 nucleotides BPHYT_RS16985 and BPHYT_RS16990 overlap by 4 nucleotides
BPHYT_RS16980: BPHYT_RS16980 - ribonuclease II, at 3,850,091 to 3,852,208
_RS16980
BPHYT_RS16985: BPHYT_RS16985 - esterase, at 3,852,415 to 3,853,017
_RS16985
BPHYT_RS16990: BPHYT_RS16990 - UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase, at 3,853,014 to 3,854,408
_RS16990
Position (kb)
3852
3853
3854 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3851.674 kb on - strand, within BPHYT_RS16980 at 3852.214 kb on - strand at 3852.214 kb on - strand at 3852.214 kb on - strand at 3852.301 kb on + strand at 3852.301 kb on + strand at 3852.396 kb on - strand at 3852.485 kb on + strand, within BPHYT_RS16985 at 3852.485 kb on + strand, within BPHYT_RS16985 at 3852.485 kb on + strand, within BPHYT_RS16985 at 3852.531 kb on + strand, within BPHYT_RS16985 at 3852.532 kb on - strand, within BPHYT_RS16985 at 3852.532 kb on - strand, within BPHYT_RS16985 at 3852.605 kb on + strand, within BPHYT_RS16985 at 3852.608 kb on + strand, within BPHYT_RS16985 at 3852.806 kb on + strand, within BPHYT_RS16985 at 3852.807 kb on - strand, within BPHYT_RS16985 at 3853.008 kb on - strand at 3853.008 kb on - strand at 3853.008 kb on - strand at 3853.242 kb on + strand, within BPHYT_RS16990 at 3853.417 kb on + strand, within BPHYT_RS16990 at 3853.417 kb on + strand, within BPHYT_RS16990 at 3853.418 kb on - strand, within BPHYT_RS16990 at 3853.612 kb on + strand, within BPHYT_RS16990 at 3853.613 kb on - strand, within BPHYT_RS16990
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 10 remove 3,851,674 - BPHYT_RS16980 0.75 -1.0 3,852,214 - -3.3 3,852,214 - -0.2 3,852,214 - -2.9 3,852,301 + -2.6 3,852,301 + -1.5 3,852,396 - +0.8 3,852,485 + BPHYT_RS16985 0.12 -1.5 3,852,485 + BPHYT_RS16985 0.12 -3.6 3,852,485 + BPHYT_RS16985 0.12 -1.8 3,852,531 + BPHYT_RS16985 0.19 -1.5 3,852,532 - BPHYT_RS16985 0.19 -0.7 3,852,532 - BPHYT_RS16985 0.19 +0.1 3,852,605 + BPHYT_RS16985 0.32 -1.0 3,852,608 + BPHYT_RS16985 0.32 -2.0 3,852,806 + BPHYT_RS16985 0.65 -2.6 3,852,807 - BPHYT_RS16985 0.65 -0.7 3,853,008 - -0.7 3,853,008 - -1.4 3,853,008 - -0.6 3,853,242 + BPHYT_RS16990 0.16 -0.9 3,853,417 + BPHYT_RS16990 0.29 -2.3 3,853,417 + BPHYT_RS16990 0.29 -2.7 3,853,418 - BPHYT_RS16990 0.29 +0.2 3,853,612 + BPHYT_RS16990 0.43 -1.4 3,853,613 - BPHYT_RS16990 0.43 +1.4
Or see this region's nucleotide sequence