Experiment: 36hr PHYTO10 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS13600 and BPHYT_RS13605 are separated by 741 nucleotides BPHYT_RS13605 and BPHYT_RS13610 are separated by 63 nucleotides BPHYT_RS13610 and BPHYT_RS13615 are separated by 46 nucleotides
BPHYT_RS13600: BPHYT_RS13600 - thioesterase, at 3,101,849 to 3,102,421
_RS13600
BPHYT_RS13605: BPHYT_RS13605 - tRNA-Pro, at 3,103,163 to 3,103,239
_RS13605
BPHYT_RS13610: BPHYT_RS13610 - MerR family transcriptional regulator, at 3,103,303 to 3,103,716
_RS13610
BPHYT_RS13615: BPHYT_RS13615 - integration host factor subunit alpha, at 3,103,763 to 3,104,173
_RS13615
Position (kb)
3103
3104 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3102.306 kb on - strand, within BPHYT_RS13600 at 3102.350 kb on - strand, within BPHYT_RS13600 at 3102.390 kb on - strand at 3102.390 kb on - strand at 3102.474 kb on + strand at 3102.517 kb on + strand at 3102.517 kb on + strand at 3102.519 kb on + strand at 3102.529 kb on - strand at 3102.643 kb on + strand at 3102.761 kb on + strand at 3102.780 kb on + strand at 3102.780 kb on + strand at 3102.781 kb on - strand at 3102.837 kb on - strand at 3102.905 kb on + strand at 3102.921 kb on - strand at 3103.155 kb on - strand at 3103.214 kb on - strand, within BPHYT_RS13605 at 3103.256 kb on - strand at 3103.256 kb on - strand at 3103.325 kb on - strand at 3103.500 kb on - strand, within BPHYT_RS13610 at 3103.548 kb on - strand, within BPHYT_RS13610 at 3103.551 kb on - strand, within BPHYT_RS13610 at 3103.551 kb on - strand, within BPHYT_RS13610 at 3103.665 kb on - strand, within BPHYT_RS13610 at 3104.177 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 10 remove 3,102,306 - BPHYT_RS13600 0.80 +0.9 3,102,350 - BPHYT_RS13600 0.87 -0.4 3,102,390 - -0.3 3,102,390 - -0.7 3,102,474 + -0.3 3,102,517 + -0.9 3,102,517 + +0.8 3,102,519 + -0.7 3,102,529 - +0.1 3,102,643 + +0.7 3,102,761 + +0.5 3,102,780 + -0.9 3,102,780 + +0.6 3,102,781 - -0.7 3,102,837 - -0.7 3,102,905 + -0.8 3,102,921 - +1.8 3,103,155 - +0.6 3,103,214 - BPHYT_RS13605 0.66 -2.3 3,103,256 - -0.4 3,103,256 - -0.4 3,103,325 - +2.5 3,103,500 - BPHYT_RS13610 0.48 -1.5 3,103,548 - BPHYT_RS13610 0.59 -0.1 3,103,551 - BPHYT_RS13610 0.60 +2.5 3,103,551 - BPHYT_RS13610 0.60 +1.5 3,103,665 - BPHYT_RS13610 0.87 +0.1 3,104,177 + +2.0
Or see this region's nucleotide sequence