Experiment: 36hr PHYTO10 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10875 and BPHYT_RS10880 are separated by 169 nucleotides BPHYT_RS10880 and BPHYT_RS10885 overlap by 11 nucleotides BPHYT_RS10885 and BPHYT_RS10890 are separated by 12 nucleotides BPHYT_RS10890 and BPHYT_RS10895 are separated by 179 nucleotides
BPHYT_RS10875: BPHYT_RS10875 - transporter, at 2,473,874 to 2,476,924
_RS10875
BPHYT_RS10880: BPHYT_RS10880 - hopanoid biosynthesis associated protein HpnK, at 2,477,094 to 2,477,918
_RS10880
BPHYT_RS10885: BPHYT_RS10885 - glycosyl transferase, at 2,477,908 to 2,479,215
_RS10885
BPHYT_RS10890: BPHYT_RS10890 - hypothetical protein, at 2,479,228 to 2,479,578
_RS10890
BPHYT_RS10895: BPHYT_RS10895 - transcriptional regulator, at 2,479,758 to 2,480,417
_RS10895
Position (kb)
2477
2478
2479
2480 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2477.095 kb on + strand at 2478.079 kb on - strand, within BPHYT_RS10885 at 2478.331 kb on - strand, within BPHYT_RS10885 at 2478.971 kb on + strand, within BPHYT_RS10885 at 2479.080 kb on - strand, within BPHYT_RS10885 at 2479.645 kb on - strand at 2479.709 kb on - strand at 2479.757 kb on + strand at 2479.758 kb on - strand at 2479.758 kb on - strand at 2479.765 kb on + strand at 2479.765 kb on + strand at 2480.050 kb on - strand, within BPHYT_RS10895
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 10 remove 2,477,095 + -2.8 2,478,079 - BPHYT_RS10885 0.13 +0.9 2,478,331 - BPHYT_RS10885 0.32 +0.3 2,478,971 + BPHYT_RS10885 0.81 -0.7 2,479,080 - BPHYT_RS10885 0.90 -0.9 2,479,645 - +1.1 2,479,709 - -0.1 2,479,757 + +0.4 2,479,758 - -0.7 2,479,758 - +2.5 2,479,765 + -0.7 2,479,765 + +0.2 2,480,050 - BPHYT_RS10895 0.44 -1.4
Or see this region's nucleotide sequence