Experiment: 36hr PHYTO10 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10295 and BPHYT_RS10300 overlap by 4 nucleotides BPHYT_RS10300 and BPHYT_RS10305 are separated by 995 nucleotides
BPHYT_RS10295: BPHYT_RS10295 - hypothetical protein, at 2,340,763 to 2,341,980
_RS10295
BPHYT_RS10300: BPHYT_RS10300 - racemase, at 2,341,977 to 2,342,714
_RS10300
BPHYT_RS10305: BPHYT_RS10305 - hypothetical protein, at 2,343,710 to 2,343,898
_RS10305
Position (kb)
2341
2342
2343 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2341.070 kb on - strand, within BPHYT_RS10295 at 2341.519 kb on + strand, within BPHYT_RS10295 at 2341.626 kb on + strand, within BPHYT_RS10295 at 2341.627 kb on - strand, within BPHYT_RS10295 at 2341.648 kb on + strand, within BPHYT_RS10295 at 2341.648 kb on + strand, within BPHYT_RS10295 at 2341.648 kb on + strand, within BPHYT_RS10295 at 2341.649 kb on - strand, within BPHYT_RS10295 at 2341.649 kb on - strand, within BPHYT_RS10295 at 2341.660 kb on + strand, within BPHYT_RS10295 at 2341.930 kb on + strand at 2341.930 kb on + strand at 2341.931 kb on - strand at 2342.031 kb on - strand at 2342.031 kb on - strand at 2342.358 kb on - strand, within BPHYT_RS10300 at 2342.392 kb on - strand, within BPHYT_RS10300 at 2342.602 kb on - strand, within BPHYT_RS10300 at 2342.602 kb on - strand, within BPHYT_RS10300 at 2342.689 kb on - strand at 2342.691 kb on - strand at 2342.755 kb on - strand at 2343.284 kb on + strand at 2343.381 kb on + strand at 2343.381 kb on + strand at 2343.386 kb on - strand at 2343.386 kb on - strand at 2343.685 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 10 remove 2,341,070 - BPHYT_RS10295 0.25 -0.2 2,341,519 + BPHYT_RS10295 0.62 -1.4 2,341,626 + BPHYT_RS10295 0.71 -0.6 2,341,627 - BPHYT_RS10295 0.71 -1.1 2,341,648 + BPHYT_RS10295 0.73 -0.4 2,341,648 + BPHYT_RS10295 0.73 +0.5 2,341,648 + BPHYT_RS10295 0.73 -0.0 2,341,649 - BPHYT_RS10295 0.73 +0.5 2,341,649 - BPHYT_RS10295 0.73 -0.9 2,341,660 + BPHYT_RS10295 0.74 +1.3 2,341,930 + +0.3 2,341,930 + -0.9 2,341,931 - -0.5 2,342,031 - -0.7 2,342,031 - -0.3 2,342,358 - BPHYT_RS10300 0.52 -1.1 2,342,392 - BPHYT_RS10300 0.56 -1.3 2,342,602 - BPHYT_RS10300 0.85 -2.2 2,342,602 - BPHYT_RS10300 0.85 -0.9 2,342,689 - +0.9 2,342,691 - +0.2 2,342,755 - -0.3 2,343,284 + -0.1 2,343,381 + -0.7 2,343,381 + +1.8 2,343,386 - +2.1 2,343,386 - +0.1 2,343,685 + +1.8
Or see this region's nucleotide sequence