Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS10300

Experiment: 36hr PHYTO10 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS10295 and BPHYT_RS10300 overlap by 4 nucleotidesBPHYT_RS10300 and BPHYT_RS10305 are separated by 995 nucleotides BPHYT_RS10295: BPHYT_RS10295 - hypothetical protein, at 2,340,763 to 2,341,980 _RS10295 BPHYT_RS10300: BPHYT_RS10300 - racemase, at 2,341,977 to 2,342,714 _RS10300 BPHYT_RS10305: BPHYT_RS10305 - hypothetical protein, at 2,343,710 to 2,343,898 _RS10305 Position (kb) 2341 2342 2343Strain fitness (log2 ratio) -2 -1 0 1 2at 2341.070 kb on - strand, within BPHYT_RS10295at 2341.519 kb on + strand, within BPHYT_RS10295at 2341.626 kb on + strand, within BPHYT_RS10295at 2341.627 kb on - strand, within BPHYT_RS10295at 2341.648 kb on + strand, within BPHYT_RS10295at 2341.648 kb on + strand, within BPHYT_RS10295at 2341.648 kb on + strand, within BPHYT_RS10295at 2341.649 kb on - strand, within BPHYT_RS10295at 2341.649 kb on - strand, within BPHYT_RS10295at 2341.660 kb on + strand, within BPHYT_RS10295at 2341.930 kb on + strandat 2341.930 kb on + strandat 2341.931 kb on - strandat 2342.031 kb on - strandat 2342.031 kb on - strandat 2342.358 kb on - strand, within BPHYT_RS10300at 2342.392 kb on - strand, within BPHYT_RS10300at 2342.602 kb on - strand, within BPHYT_RS10300at 2342.602 kb on - strand, within BPHYT_RS10300at 2342.689 kb on - strandat 2342.691 kb on - strandat 2342.755 kb on - strandat 2343.284 kb on + strandat 2343.381 kb on + strandat 2343.381 kb on + strandat 2343.386 kb on - strandat 2343.386 kb on - strandat 2343.685 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 10
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2,341,070 - BPHYT_RS10295 0.25 -0.2
2,341,519 + BPHYT_RS10295 0.62 -1.4
2,341,626 + BPHYT_RS10295 0.71 -0.6
2,341,627 - BPHYT_RS10295 0.71 -1.1
2,341,648 + BPHYT_RS10295 0.73 -0.4
2,341,648 + BPHYT_RS10295 0.73 +0.5
2,341,648 + BPHYT_RS10295 0.73 -0.0
2,341,649 - BPHYT_RS10295 0.73 +0.5
2,341,649 - BPHYT_RS10295 0.73 -0.9
2,341,660 + BPHYT_RS10295 0.74 +1.3
2,341,930 + +0.3
2,341,930 + -0.9
2,341,931 - -0.5
2,342,031 - -0.7
2,342,031 - -0.3
2,342,358 - BPHYT_RS10300 0.52 -1.1
2,342,392 - BPHYT_RS10300 0.56 -1.3
2,342,602 - BPHYT_RS10300 0.85 -2.2
2,342,602 - BPHYT_RS10300 0.85 -0.9
2,342,689 - +0.9
2,342,691 - +0.2
2,342,755 - -0.3
2,343,284 + -0.1
2,343,381 + -0.7
2,343,381 + +1.8
2,343,386 - +2.1
2,343,386 - +0.1
2,343,685 + +1.8

Or see this region's nucleotide sequence