Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS07645

Experiment: 36hr PHYTO10 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS07635 and BPHYT_RS07640 are separated by 56 nucleotidesBPHYT_RS07640 and BPHYT_RS07645 are separated by 128 nucleotidesBPHYT_RS07645 and BPHYT_RS07650 overlap by 4 nucleotides BPHYT_RS07635: BPHYT_RS07635 - alkanesulfonate monooxygenase, at 1,738,248 to 1,739,327 _RS07635 BPHYT_RS07640: BPHYT_RS07640 - hypothetical protein, at 1,739,384 to 1,740,163 _RS07640 BPHYT_RS07645: BPHYT_RS07645 - cysteine dioxygenase, at 1,740,292 to 1,740,900 _RS07645 BPHYT_RS07650: BPHYT_RS07650 - sulfurtransferase, at 1,740,897 to 1,742,498 _RS07650 Position (kb) 1740 1741Strain fitness (log2 ratio) -1 0 1at 1739.820 kb on - strand, within BPHYT_RS07640at 1739.979 kb on - strand, within BPHYT_RS07640at 1740.478 kb on + strand, within BPHYT_RS07645at 1740.648 kb on + strand, within BPHYT_RS07645at 1740.744 kb on + strand, within BPHYT_RS07645at 1740.767 kb on - strand, within BPHYT_RS07645at 1740.866 kb on + strandat 1740.866 kb on + strandat 1741.161 kb on + strand, within BPHYT_RS07650at 1741.162 kb on - strand, within BPHYT_RS07650at 1741.752 kb on + strand, within BPHYT_RS07650

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 10
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1,739,820 - BPHYT_RS07640 0.56 +0.3
1,739,979 - BPHYT_RS07640 0.76 +1.1
1,740,478 + BPHYT_RS07645 0.31 +0.1
1,740,648 + BPHYT_RS07645 0.58 +0.3
1,740,744 + BPHYT_RS07645 0.74 +0.4
1,740,767 - BPHYT_RS07645 0.78 -0.1
1,740,866 + +0.6
1,740,866 + -1.3
1,741,161 + BPHYT_RS07650 0.16 +0.1
1,741,162 - BPHYT_RS07650 0.17 -0.1
1,741,752 + BPHYT_RS07650 0.53 -0.2

Or see this region's nucleotide sequence