Experiment: 36hr PHYTO10 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS06405 and BPHYT_RS06410 are separated by 247 nucleotides BPHYT_RS06410 and BPHYT_RS06415 are separated by 484 nucleotides
BPHYT_RS06405: BPHYT_RS06405 - phosphoribosylamine--glycine ligase, at 1,457,486 to 1,458,784
_RS06405
BPHYT_RS06410: BPHYT_RS06410 - transcriptional regulator, at 1,459,032 to 1,459,760
_RS06410
BPHYT_RS06415: BPHYT_RS06415 - uracil phosphoribosyltransferase, at 1,460,245 to 1,460,895
_RS06415
Position (kb)
1459
1460 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1458.390 kb on + strand, within BPHYT_RS06405 at 1458.391 kb on - strand, within BPHYT_RS06405 at 1458.427 kb on - strand, within BPHYT_RS06405 at 1458.699 kb on + strand at 1458.700 kb on - strand at 1458.700 kb on - strand at 1458.776 kb on + strand at 1458.776 kb on + strand at 1458.801 kb on + strand at 1458.810 kb on + strand at 1459.058 kb on + strand at 1459.059 kb on - strand at 1459.493 kb on - strand, within BPHYT_RS06410 at 1459.516 kb on + strand, within BPHYT_RS06410 at 1459.569 kb on - strand, within BPHYT_RS06410 at 1459.725 kb on - strand at 1459.764 kb on + strand at 1459.801 kb on - strand at 1460.172 kb on + strand at 1460.173 kb on - strand at 1460.300 kb on + strand at 1460.300 kb on + strand at 1460.300 kb on + strand at 1460.300 kb on + strand at 1460.301 kb on - strand at 1460.301 kb on - strand at 1460.301 kb on - strand at 1460.301 kb on - strand at 1460.339 kb on + strand, within BPHYT_RS06415 at 1460.540 kb on + strand, within BPHYT_RS06415 at 1460.754 kb on - strand, within BPHYT_RS06415
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 10 remove 1,458,390 + BPHYT_RS06405 0.70 -0.3 1,458,391 - BPHYT_RS06405 0.70 +0.8 1,458,427 - BPHYT_RS06405 0.72 +0.4 1,458,699 + -0.1 1,458,700 - -0.1 1,458,700 - +0.6 1,458,776 + +0.1 1,458,776 + +1.9 1,458,801 + +0.4 1,458,810 + +0.9 1,459,058 + +0.0 1,459,059 - -0.9 1,459,493 - BPHYT_RS06410 0.63 -0.4 1,459,516 + BPHYT_RS06410 0.66 -3.2 1,459,569 - BPHYT_RS06410 0.74 -2.1 1,459,725 - -0.5 1,459,764 + -1.1 1,459,801 - -1.0 1,460,172 + +0.1 1,460,173 - +0.1 1,460,300 + -0.1 1,460,300 + -2.4 1,460,300 + -0.5 1,460,300 + -0.2 1,460,301 - -1.1 1,460,301 - -0.3 1,460,301 - -0.2 1,460,301 - -0.2 1,460,339 + BPHYT_RS06415 0.14 -0.1 1,460,540 + BPHYT_RS06415 0.45 -2.9 1,460,754 - BPHYT_RS06415 0.78 -1.6
Or see this region's nucleotide sequence