Experiment: 36hr PHYTO10 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS04435 and BPHYT_RS04440 are separated by 8 nucleotides BPHYT_RS04440 and BPHYT_RS04445 are separated by 108 nucleotides BPHYT_RS04445 and BPHYT_RS04450 are separated by 224 nucleotides
BPHYT_RS04435: BPHYT_RS04435 - ABC transporter, at 1,024,939 to 1,026,879
_RS04435
BPHYT_RS04440: BPHYT_RS04440 - ABC transporter permease, at 1,026,888 to 1,027,763
_RS04440
BPHYT_RS04445: BPHYT_RS04445 - amino acid ABC transporter substrate-binding protein, at 1,027,872 to 1,029,089
_RS04445
BPHYT_RS04450: BPHYT_RS04450 - UDP-glucose 4-epimerase, at 1,029,314 to 1,030,336
_RS04450
Position (kb) 1027
1028
1029
1030 Strain fitness (log2 ratio) -2
-1
0
1 at 1026.979 kb on + strand, within BPHYT_RS04440 at 1026.995 kb on - strand, within BPHYT_RS04440 at 1027.711 kb on + strand at 1027.711 kb on + strand at 1027.739 kb on - strand at 1027.739 kb on - strand at 1027.835 kb on - strand at 1027.934 kb on - strand at 1027.940 kb on - strand at 1028.132 kb on - strand, within BPHYT_RS04445 at 1028.304 kb on - strand at 1028.306 kb on - strand, within BPHYT_RS04445 at 1028.555 kb on - strand, within BPHYT_RS04445 at 1028.666 kb on - strand, within BPHYT_RS04445 at 1028.666 kb on - strand, within BPHYT_RS04445 at 1028.666 kb on - strand, within BPHYT_RS04445 at 1028.789 kb on - strand, within BPHYT_RS04445 at 1029.025 kb on + strand at 1029.197 kb on - strand at 1029.219 kb on + strand at 1029.528 kb on + strand, within BPHYT_RS04450 at 1029.529 kb on - strand, within BPHYT_RS04450 at 1029.631 kb on - strand, within BPHYT_RS04450 at 1030.028 kb on - strand, within BPHYT_RS04450 at 1030.036 kb on - strand, within BPHYT_RS04450
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 10 remove 1,026,979 + BPHYT_RS04440 0.10 -1.0 1,026,995 - BPHYT_RS04440 0.12 -0.5 1,027,711 + +1.5 1,027,711 + +0.2 1,027,739 - -0.9 1,027,739 - -1.7 1,027,835 - +0.1 1,027,934 - +0.6 1,027,940 - -0.6 1,028,132 - BPHYT_RS04445 0.21 +0.7 1,028,304 - +1.2 1,028,306 - BPHYT_RS04445 0.36 -1.5 1,028,555 - BPHYT_RS04445 0.56 -0.3 1,028,666 - BPHYT_RS04445 0.65 -1.1 1,028,666 - BPHYT_RS04445 0.65 -2.4 1,028,666 - BPHYT_RS04445 0.65 -1.4 1,028,789 - BPHYT_RS04445 0.75 -2.2 1,029,025 + -0.5 1,029,197 - +0.2 1,029,219 + +0.3 1,029,528 + BPHYT_RS04450 0.21 -0.2 1,029,529 - BPHYT_RS04450 0.21 -0.6 1,029,631 - BPHYT_RS04450 0.31 -0.1 1,030,028 - BPHYT_RS04450 0.70 -0.1 1,030,036 - BPHYT_RS04450 0.71 -0.7
Or see this region's nucleotide sequence