Experiment: 36hr PHYTO10 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS03250 and BPHYT_RS03255 are separated by 19 nucleotides BPHYT_RS03255 and BPHYT_RS03260 are separated by 160 nucleotides BPHYT_RS03260 and BPHYT_RS03265 are separated by 195 nucleotides BPHYT_RS03265 and BPHYT_RS03270 are separated by 68 nucleotides
BPHYT_RS03250: BPHYT_RS03250 - diguanylate cyclase, at 730,026 to 733,136
_RS03250
BPHYT_RS03255: BPHYT_RS03255 - glutathione S-transferase, at 733,156 to 733,770
_RS03255
BPHYT_RS03260: BPHYT_RS03260 - glycine cleavage system regulatory protein, at 733,931 to 734,860
_RS03260
BPHYT_RS03265: BPHYT_RS03265 - hypothetical protein, at 735,056 to 735,394
_RS03265
BPHYT_RS03270: BPHYT_RS03270 - acyl-CoA hydrolase, at 735,463 to 735,858
_RS03270
Position (kb) 733
734
735 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 733.056 kb on + strand at 733.057 kb on - strand at 733.057 kb on - strand at 733.057 kb on - strand at 733.111 kb on + strand at 733.112 kb on - strand at 733.566 kb on - strand, within BPHYT_RS03255 at 733.661 kb on + strand, within BPHYT_RS03255 at 733.662 kb on - strand, within BPHYT_RS03255 at 733.709 kb on - strand at 733.791 kb on + strand at 733.792 kb on - strand at 733.869 kb on + strand at 733.869 kb on + strand at 733.902 kb on + strand at 733.903 kb on - strand at 734.291 kb on + strand, within BPHYT_RS03260 at 734.291 kb on + strand, within BPHYT_RS03260 at 734.729 kb on + strand, within BPHYT_RS03260 at 734.813 kb on + strand at 734.813 kb on + strand at 734.813 kb on + strand at 734.813 kb on + strand at 734.814 kb on - strand at 734.814 kb on - strand at 734.866 kb on - strand at 734.878 kb on - strand at 734.908 kb on - strand at 734.908 kb on - strand at 735.105 kb on + strand, within BPHYT_RS03265 at 735.105 kb on + strand, within BPHYT_RS03265 at 735.106 kb on - strand, within BPHYT_RS03265 at 735.403 kb on + strand at 735.403 kb on + strand at 735.404 kb on - strand at 735.404 kb on - strand at 735.404 kb on - strand at 735.404 kb on - strand at 735.515 kb on + strand, within BPHYT_RS03270 at 735.516 kb on - strand, within BPHYT_RS03270 at 735.516 kb on - strand, within BPHYT_RS03270 at 735.576 kb on - strand, within BPHYT_RS03270 at 735.604 kb on - strand, within BPHYT_RS03270 at 735.629 kb on + strand, within BPHYT_RS03270 at 735.630 kb on - strand, within BPHYT_RS03270 at 735.849 kb on + strand at 735.849 kb on + strand at 735.849 kb on + strand at 735.850 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 10 remove 733,056 + -1.7 733,057 - -1.1 733,057 - +0.2 733,057 - -0.6 733,111 + +0.3 733,112 - +0.2 733,566 - BPHYT_RS03255 0.67 +1.7 733,661 + BPHYT_RS03255 0.82 -0.8 733,662 - BPHYT_RS03255 0.82 -1.2 733,709 - -0.1 733,791 + -0.9 733,792 - -0.5 733,869 + -1.2 733,869 + -0.9 733,902 + -0.7 733,903 - -0.1 734,291 + BPHYT_RS03260 0.39 -1.5 734,291 + BPHYT_RS03260 0.39 -1.2 734,729 + BPHYT_RS03260 0.86 -0.0 734,813 + -1.6 734,813 + +0.1 734,813 + -2.0 734,813 + -1.4 734,814 - +0.9 734,814 - -0.5 734,866 - -1.5 734,878 - -0.4 734,908 - -0.0 734,908 - -0.7 735,105 + BPHYT_RS03265 0.14 -1.6 735,105 + BPHYT_RS03265 0.14 +0.8 735,106 - BPHYT_RS03265 0.15 +1.2 735,403 + +0.0 735,403 + -0.9 735,404 - +0.2 735,404 - -1.1 735,404 - -4.0 735,404 - -1.0 735,515 + BPHYT_RS03270 0.13 -0.2 735,516 - BPHYT_RS03270 0.13 -0.5 735,516 - BPHYT_RS03270 0.13 -1.1 735,576 - BPHYT_RS03270 0.29 +0.3 735,604 - BPHYT_RS03270 0.36 -0.0 735,629 + BPHYT_RS03270 0.42 -0.2 735,630 - BPHYT_RS03270 0.42 +1.1 735,849 + -3.6 735,849 + -1.0 735,849 + -0.7 735,850 - +0.3
Or see this region's nucleotide sequence