Experiment: 36hr PHYTO4 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS32135 and BPHYT_RS32140 are separated by 51 nucleotides BPHYT_RS32140 and BPHYT_RS32145 overlap by 4 nucleotides
BPHYT_RS32135: BPHYT_RS32135 - DNA primase, at 2,781,706 to 2,783,229
_RS32135
BPHYT_RS32140: BPHYT_RS32140 - DNA replication protein, at 2,783,281 to 2,785,146
_RS32140
BPHYT_RS32145: BPHYT_RS32145 - type IV secretory pathway protein VirD4, at 2,785,143 to 2,786,894
_RS32145
Position (kb)
2783
2784
2785
2786 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2782.328 kb on + strand, within BPHYT_RS32135 at 2782.328 kb on + strand, within BPHYT_RS32135 at 2782.328 kb on + strand, within BPHYT_RS32135 at 2782.398 kb on - strand, within BPHYT_RS32135 at 2782.564 kb on + strand, within BPHYT_RS32135 at 2782.564 kb on + strand, within BPHYT_RS32135 at 2782.564 kb on + strand, within BPHYT_RS32135 at 2782.565 kb on - strand, within BPHYT_RS32135 at 2782.565 kb on - strand, within BPHYT_RS32135 at 2782.570 kb on + strand, within BPHYT_RS32135 at 2782.571 kb on - strand, within BPHYT_RS32135 at 2782.717 kb on + strand, within BPHYT_RS32135 at 2782.718 kb on - strand, within BPHYT_RS32135 at 2782.883 kb on + strand, within BPHYT_RS32135 at 2783.663 kb on - strand, within BPHYT_RS32140 at 2783.710 kb on + strand, within BPHYT_RS32140 at 2783.746 kb on + strand, within BPHYT_RS32140 at 2783.747 kb on - strand, within BPHYT_RS32140 at 2784.058 kb on + strand, within BPHYT_RS32140 at 2784.058 kb on + strand, within BPHYT_RS32140 at 2784.629 kb on + strand, within BPHYT_RS32140 at 2784.630 kb on - strand, within BPHYT_RS32140 at 2784.708 kb on - strand, within BPHYT_RS32140 at 2785.140 kb on + strand at 2785.140 kb on + strand at 2785.140 kb on + strand at 2785.141 kb on - strand at 2785.141 kb on - strand at 2785.328 kb on - strand, within BPHYT_RS32145 at 2785.408 kb on + strand, within BPHYT_RS32145 at 2785.409 kb on - strand, within BPHYT_RS32145 at 2785.585 kb on + strand, within BPHYT_RS32145 at 2785.826 kb on - strand, within BPHYT_RS32145 at 2785.859 kb on + strand, within BPHYT_RS32145 at 2785.859 kb on + strand, within BPHYT_RS32145 at 2785.946 kb on - strand, within BPHYT_RS32145 at 2786.135 kb on - strand, within BPHYT_RS32145
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 0.1 remove 2,782,328 + BPHYT_RS32135 0.41 -0.7 2,782,328 + BPHYT_RS32135 0.41 -1.2 2,782,328 + BPHYT_RS32135 0.41 +0.1 2,782,398 - BPHYT_RS32135 0.45 +1.6 2,782,564 + BPHYT_RS32135 0.56 +1.8 2,782,564 + BPHYT_RS32135 0.56 +0.4 2,782,564 + BPHYT_RS32135 0.56 +0.4 2,782,565 - BPHYT_RS32135 0.56 -0.0 2,782,565 - BPHYT_RS32135 0.56 -0.9 2,782,570 + BPHYT_RS32135 0.57 -0.1 2,782,571 - BPHYT_RS32135 0.57 +0.4 2,782,717 + BPHYT_RS32135 0.66 -0.2 2,782,718 - BPHYT_RS32135 0.66 -0.4 2,782,883 + BPHYT_RS32135 0.77 -0.4 2,783,663 - BPHYT_RS32140 0.20 -0.5 2,783,710 + BPHYT_RS32140 0.23 +0.2 2,783,746 + BPHYT_RS32140 0.25 -0.6 2,783,747 - BPHYT_RS32140 0.25 +0.3 2,784,058 + BPHYT_RS32140 0.42 -0.2 2,784,058 + BPHYT_RS32140 0.42 -0.2 2,784,629 + BPHYT_RS32140 0.72 -0.4 2,784,630 - BPHYT_RS32140 0.72 +0.9 2,784,708 - BPHYT_RS32140 0.76 +1.0 2,785,140 + +1.8 2,785,140 + +1.5 2,785,140 + -0.5 2,785,141 - +2.4 2,785,141 - +1.4 2,785,328 - BPHYT_RS32145 0.11 +0.6 2,785,408 + BPHYT_RS32145 0.15 -0.0 2,785,409 - BPHYT_RS32145 0.15 +0.5 2,785,585 + BPHYT_RS32145 0.25 -0.4 2,785,826 - BPHYT_RS32145 0.39 +0.5 2,785,859 + BPHYT_RS32145 0.41 -1.8 2,785,859 + BPHYT_RS32145 0.41 +0.6 2,785,946 - BPHYT_RS32145 0.46 +1.0 2,786,135 - BPHYT_RS32145 0.57 +1.5
Or see this region's nucleotide sequence