Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS31545

Experiment: 36hr PHYTO4 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS31540 and BPHYT_RS31545 are separated by 125 nucleotidesBPHYT_RS31545 and BPHYT_RS31550 are separated by 441 nucleotides BPHYT_RS31540: BPHYT_RS31540 - chloride channel protein, at 2,629,423 to 2,630,748 _RS31540 BPHYT_RS31545: BPHYT_RS31545 - membrane protein, at 2,630,874 to 2,631,539 _RS31545 BPHYT_RS31550: BPHYT_RS31550 - hypothetical protein, at 2,631,981 to 2,632,217 _RS31550 Position (kb) 2630 2631 2632Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 2630.085 kb on - strand, within BPHYT_RS31540at 2630.546 kb on - strand, within BPHYT_RS31540at 2630.548 kb on - strand, within BPHYT_RS31540at 2630.685 kb on - strandat 2630.781 kb on - strandat 2631.318 kb on - strand, within BPHYT_RS31545at 2631.423 kb on + strand, within BPHYT_RS31545at 2631.424 kb on - strand, within BPHYT_RS31545at 2631.424 kb on - strand, within BPHYT_RS31545at 2631.424 kb on - strand, within BPHYT_RS31545at 2631.456 kb on + strand, within BPHYT_RS31545at 2631.539 kb on + strandat 2631.542 kb on - strandat 2631.542 kb on - strandat 2631.542 kb on - strandat 2631.653 kb on + strandat 2631.654 kb on - strandat 2631.833 kb on - strandat 2632.122 kb on + strand, within BPHYT_RS31550at 2632.123 kb on - strand, within BPHYT_RS31550at 2632.137 kb on - strand, within BPHYT_RS31550at 2632.155 kb on + strand, within BPHYT_RS31550at 2632.223 kb on - strandat 2632.449 kb on + strandat 2632.449 kb on + strandat 2632.450 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 0.1
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2,630,085 - BPHYT_RS31540 0.50 -1.2
2,630,546 - BPHYT_RS31540 0.85 -0.4
2,630,548 - BPHYT_RS31540 0.85 -0.3
2,630,685 - -0.4
2,630,781 - -1.1
2,631,318 - BPHYT_RS31545 0.67 -1.9
2,631,423 + BPHYT_RS31545 0.82 +1.8
2,631,424 - BPHYT_RS31545 0.83 +1.9
2,631,424 - BPHYT_RS31545 0.83 -1.8
2,631,424 - BPHYT_RS31545 0.83 +1.3
2,631,456 + BPHYT_RS31545 0.87 -0.2
2,631,539 + -0.4
2,631,542 - -1.2
2,631,542 - +2.4
2,631,542 - -0.8
2,631,653 + -0.9
2,631,654 - -0.6
2,631,833 - -1.1
2,632,122 + BPHYT_RS31550 0.59 +4.4
2,632,123 - BPHYT_RS31550 0.60 +0.5
2,632,137 - BPHYT_RS31550 0.66 +0.3
2,632,155 + BPHYT_RS31550 0.73 +0.4
2,632,223 - +1.2
2,632,449 + -0.9
2,632,449 + -1.2
2,632,450 - -0.3

Or see this region's nucleotide sequence