Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS24065

Experiment: 36hr PHYTO4 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS24060 and BPHYT_RS24065 are separated by 25 nucleotidesBPHYT_RS24065 and BPHYT_RS24070 are separated by 204 nucleotides BPHYT_RS24060: BPHYT_RS24060 - FAD-linked oxidase, at 961,627 to 963,039 _RS24060 BPHYT_RS24065: BPHYT_RS24065 - glucose-methanol-choline oxidoreductase, at 963,065 to 966,523 _RS24065 BPHYT_RS24070: BPHYT_RS24070 - hypothetical protein, at 966,728 to 967,519 _RS24070 Position (kb) 963 964 965 966 967Strain fitness (log2 ratio) -1 0 1 2at 962.344 kb on + strand, within BPHYT_RS24060at 962.431 kb on + strand, within BPHYT_RS24060at 963.082 kb on - strandat 963.457 kb on - strand, within BPHYT_RS24065at 963.628 kb on + strand, within BPHYT_RS24065at 964.362 kb on + strand, within BPHYT_RS24065at 965.107 kb on - strand, within BPHYT_RS24065at 965.107 kb on - strand, within BPHYT_RS24065at 965.452 kb on - strand, within BPHYT_RS24065at 966.324 kb on + strandat 967.154 kb on - strand, within BPHYT_RS24070at 967.227 kb on + strand, within BPHYT_RS24070at 967.445 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 0.1
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962,344 + BPHYT_RS24060 0.51 +0.3
962,431 + BPHYT_RS24060 0.57 -0.0
963,082 - +0.4
963,457 - BPHYT_RS24065 0.11 -0.7
963,628 + BPHYT_RS24065 0.16 -0.3
964,362 + BPHYT_RS24065 0.37 -0.7
965,107 - BPHYT_RS24065 0.59 +1.0
965,107 - BPHYT_RS24065 0.59 -1.2
965,452 - BPHYT_RS24065 0.69 -0.3
966,324 + +0.3
967,154 - BPHYT_RS24070 0.54 +0.4
967,227 + BPHYT_RS24070 0.63 +0.1
967,445 - +2.3

Or see this region's nucleotide sequence