Experiment: 36hr PHYTO4 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS19975 and BPHYT_RS19980 are separated by 29 nucleotides BPHYT_RS19980 and BPHYT_RS19985 are separated by 2 nucleotides BPHYT_RS19985 and BPHYT_RS19990 are separated by 41 nucleotides
BPHYT_RS19975: BPHYT_RS19975 - IclR family transcriptional regulator, at 24,912 to 25,676
_RS19975
BPHYT_RS19980: BPHYT_RS19980 - citrate synthase, at 25,706 to 26,530
_RS19980
BPHYT_RS19985: BPHYT_RS19985 - CoA-transferase, at 26,533 to 27,732
_RS19985
BPHYT_RS19990: BPHYT_RS19990 - MFS transporter, at 27,774 to 29,207
_RS19990
Position (kb) 26
27
28 Strain fitness (log2 ratio) -1
0
1
2 at 25.674 kb on + strand at 25.737 kb on + strand at 26.172 kb on + strand, within BPHYT_RS19980 at 26.172 kb on + strand, within BPHYT_RS19980 at 26.173 kb on - strand, within BPHYT_RS19980 at 27.308 kb on + strand, within BPHYT_RS19985 at 27.663 kb on - strand at 27.694 kb on + strand at 27.695 kb on - strand at 28.661 kb on - strand, within BPHYT_RS19990 at 28.726 kb on + strand, within BPHYT_RS19990 at 28.726 kb on + strand, within BPHYT_RS19990 at 28.726 kb on + strand, within BPHYT_RS19990 at 28.727 kb on - strand, within BPHYT_RS19990 at 28.727 kb on - strand, within BPHYT_RS19990
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 0.1 remove 25,674 + +2.4 25,737 + +0.6 26,172 + BPHYT_RS19980 0.56 -1.3 26,172 + BPHYT_RS19980 0.56 +0.1 26,173 - BPHYT_RS19980 0.57 -0.8 27,308 + BPHYT_RS19985 0.65 -0.8 27,663 - -0.9 27,694 + -1.1 27,695 - +0.2 28,661 - BPHYT_RS19990 0.62 -0.8 28,726 + BPHYT_RS19990 0.66 -0.4 28,726 + BPHYT_RS19990 0.66 -0.5 28,726 + BPHYT_RS19990 0.66 -1.6 28,727 - BPHYT_RS19990 0.66 -0.6 28,727 - BPHYT_RS19990 0.66 -1.4
Or see this region's nucleotide sequence