Experiment: 36hr PHYTO4 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS13945 and BPHYT_RS13950 overlap by 8 nucleotides BPHYT_RS13950 and BPHYT_RS13955 are separated by 4 nucleotides BPHYT_RS13955 and BPHYT_RS13960 are separated by 154 nucleotides
BPHYT_RS13945: BPHYT_RS13945 - branched-chain amino acid ABC transporter permease, at 3,185,338 to 3,186,630
_RS13945
BPHYT_RS13950: BPHYT_RS13950 - branched-chain amino acid ABC transporter permease, at 3,186,623 to 3,187,561
_RS13950
BPHYT_RS13955: BPHYT_RS13955 - thioesterase, at 3,187,566 to 3,188,015
_RS13955
BPHYT_RS13960: BPHYT_RS13960 - ADP-ribose pyrophosphatase, at 3,188,170 to 3,188,700
_RS13960
Position (kb)
3187
3188
3189 Strain fitness (log2 ratio)
-2
-1
0
1 at 3187.165 kb on - strand, within BPHYT_RS13950 at 3187.258 kb on - strand, within BPHYT_RS13950 at 3187.419 kb on + strand, within BPHYT_RS13950 at 3187.419 kb on + strand, within BPHYT_RS13950 at 3187.420 kb on - strand, within BPHYT_RS13950 at 3187.657 kb on + strand, within BPHYT_RS13955 at 3187.657 kb on + strand, within BPHYT_RS13955 at 3187.657 kb on + strand, within BPHYT_RS13955 at 3187.658 kb on - strand, within BPHYT_RS13955 at 3187.658 kb on - strand, within BPHYT_RS13955 at 3187.801 kb on + strand, within BPHYT_RS13955 at 3187.876 kb on + strand, within BPHYT_RS13955 at 3187.900 kb on + strand, within BPHYT_RS13955 at 3187.906 kb on + strand, within BPHYT_RS13955 at 3187.906 kb on + strand, within BPHYT_RS13955 at 3187.906 kb on + strand, within BPHYT_RS13955 at 3187.907 kb on - strand, within BPHYT_RS13955 at 3187.933 kb on + strand, within BPHYT_RS13955 at 3188.090 kb on - strand at 3188.136 kb on + strand at 3188.588 kb on + strand, within BPHYT_RS13960 at 3188.740 kb on + strand at 3188.740 kb on + strand at 3188.740 kb on + strand at 3188.741 kb on - strand at 3188.741 kb on - strand at 3188.802 kb on + strand at 3188.803 kb on - strand at 3188.823 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 0.1 remove 3,187,165 - BPHYT_RS13950 0.58 +0.2 3,187,258 - BPHYT_RS13950 0.68 -2.3 3,187,419 + BPHYT_RS13950 0.85 +0.4 3,187,419 + BPHYT_RS13950 0.85 +0.4 3,187,420 - BPHYT_RS13950 0.85 -0.3 3,187,657 + BPHYT_RS13955 0.20 -1.6 3,187,657 + BPHYT_RS13955 0.20 +0.3 3,187,657 + BPHYT_RS13955 0.20 +0.4 3,187,658 - BPHYT_RS13955 0.20 -0.9 3,187,658 - BPHYT_RS13955 0.20 -0.4 3,187,801 + BPHYT_RS13955 0.52 -0.8 3,187,876 + BPHYT_RS13955 0.69 -0.3 3,187,900 + BPHYT_RS13955 0.74 -0.5 3,187,906 + BPHYT_RS13955 0.76 -0.6 3,187,906 + BPHYT_RS13955 0.76 -0.6 3,187,906 + BPHYT_RS13955 0.76 -0.2 3,187,907 - BPHYT_RS13955 0.76 -0.8 3,187,933 + BPHYT_RS13955 0.82 -0.4 3,188,090 - -0.8 3,188,136 + -1.9 3,188,588 + BPHYT_RS13960 0.79 -1.0 3,188,740 + +0.1 3,188,740 + -1.2 3,188,740 + -0.5 3,188,741 - -1.7 3,188,741 - -0.3 3,188,802 + -0.6 3,188,803 - +0.2 3,188,823 + +0.1
Or see this region's nucleotide sequence