Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS13435

Experiment: 36hr PHYTO4 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS13430 and BPHYT_RS13435 overlap by 8 nucleotidesBPHYT_RS13435 and BPHYT_RS13440 are separated by 30 nucleotidesBPHYT_RS13440 and BPHYT_RS13445 are separated by 99 nucleotidesBPHYT_RS13445 and BPHYT_RS13450 are separated by 60 nucleotides BPHYT_RS13430: BPHYT_RS13430 - radical SAM protein, at 3,066,336 to 3,067,406 _RS13430 BPHYT_RS13435: BPHYT_RS13435 - aliphatic nitrilase, at 3,067,399 to 3,068,433 _RS13435 BPHYT_RS13440: BPHYT_RS13440 - hypothetical protein, at 3,068,464 to 3,068,946 _RS13440 BPHYT_RS13445: BPHYT_RS13445 - hypothetical protein, at 3,069,046 to 3,069,246 _RS13445 BPHYT_RS13450: BPHYT_RS13450 - GntR family transcriptional regulator, at 3,069,307 to 3,070,698 _RS13450 Position (kb) 3067 3068 3069Strain fitness (log2 ratio) -1 0 1at 3066.607 kb on + strand, within BPHYT_RS13430at 3066.665 kb on - strand, within BPHYT_RS13430at 3067.935 kb on - strand, within BPHYT_RS13435at 3068.829 kb on + strand, within BPHYT_RS13440

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 0.1
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3,066,607 + BPHYT_RS13430 0.25 -0.3
3,066,665 - BPHYT_RS13430 0.31 +1.0
3,067,935 - BPHYT_RS13435 0.52 -1.7
3,068,829 + BPHYT_RS13440 0.76 -1.1

Or see this region's nucleotide sequence