Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS13210

Experiment: 36hr PHYTO4 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS13200 and BPHYT_RS13205 are separated by 40 nucleotidesBPHYT_RS13205 and BPHYT_RS13210 are separated by 30 nucleotidesBPHYT_RS13210 and BPHYT_RS13215 are separated by 440 nucleotides BPHYT_RS13200: BPHYT_RS13200 - molybdopterin binding oxidoreductase, at 3,012,230 to 3,013,021 _RS13200 BPHYT_RS13205: BPHYT_RS13205 - cytochrome B561, at 3,013,062 to 3,013,703 _RS13205 BPHYT_RS13210: BPHYT_RS13210 - hypothetical protein, at 3,013,734 to 3,014,579 _RS13210 BPHYT_RS13215: BPHYT_RS13215 - elongation factor GreAB, at 3,015,020 to 3,015,430 _RS13215 Position (kb) 3013 3014 3015Strain fitness (log2 ratio) -2 -1 0 1 2at 3013.304 kb on + strand, within BPHYT_RS13205at 3014.178 kb on - strand, within BPHYT_RS13210at 3014.215 kb on + strand, within BPHYT_RS13210at 3014.216 kb on - strand, within BPHYT_RS13210at 3014.400 kb on - strand, within BPHYT_RS13210at 3014.419 kb on + strand, within BPHYT_RS13210at 3014.420 kb on - strand, within BPHYT_RS13210at 3014.701 kb on + strandat 3014.710 kb on + strandat 3014.919 kb on + strandat 3015.395 kb on + strandat 3015.428 kb on + strandat 3015.428 kb on + strandat 3015.428 kb on + strandat 3015.429 kb on - strandat 3015.494 kb on + strandat 3015.495 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 0.1
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3,013,304 + BPHYT_RS13205 0.38 +1.0
3,014,178 - BPHYT_RS13210 0.52 -0.8
3,014,215 + BPHYT_RS13210 0.57 +0.6
3,014,216 - BPHYT_RS13210 0.57 -1.3
3,014,400 - BPHYT_RS13210 0.79 -1.8
3,014,419 + BPHYT_RS13210 0.81 -0.6
3,014,420 - BPHYT_RS13210 0.81 -0.9
3,014,701 + +0.6
3,014,710 + -0.6
3,014,919 + +2.0
3,015,395 + +0.2
3,015,428 + -0.8
3,015,428 + -0.1
3,015,428 + -0.8
3,015,429 - +1.0
3,015,494 + -1.5
3,015,495 - -0.3

Or see this region's nucleotide sequence