Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS36280

Experiment: 36hr PHYTO4 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS36270 and BPHYT_RS36275 are separated by 386 nucleotidesBPHYT_RS36275 and BPHYT_RS36280 are separated by 1 nucleotides BPHYT_RS36270: BPHYT_RS36270 - hypothetical protein, at 63,847 to 64,425 _RS36270 BPHYT_RS36275: BPHYT_RS36275 - hypothetical protein, at 64,812 to 65,195 _RS36275 BPHYT_RS36280: BPHYT_RS36280 - type IV secretion system protein VirD4, at 65,197 to 66,885 _RS36280 Position (kb) 65 66 67Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 64.588 kb on + strandat 64.823 kb on - strandat 64.931 kb on - strand, within BPHYT_RS36275at 65.107 kb on - strand, within BPHYT_RS36275at 65.107 kb on - strand, within BPHYT_RS36275at 65.196 kb on + strandat 65.224 kb on + strandat 65.224 kb on + strandat 65.224 kb on + strandat 65.224 kb on + strandat 65.224 kb on + strandat 65.225 kb on - strandat 65.294 kb on + strandat 65.366 kb on + strand, within BPHYT_RS36280at 65.367 kb on - strand, within BPHYT_RS36280at 65.419 kb on - strand, within BPHYT_RS36280at 65.458 kb on + strandat 65.532 kb on + strand, within BPHYT_RS36280at 65.532 kb on + strand, within BPHYT_RS36280at 65.532 kb on + strand, within BPHYT_RS36280at 65.641 kb on - strand, within BPHYT_RS36280at 65.802 kb on - strand, within BPHYT_RS36280at 65.891 kb on + strand, within BPHYT_RS36280at 65.972 kb on + strandat 66.006 kb on + strand, within BPHYT_RS36280at 66.036 kb on + strand, within BPHYT_RS36280at 66.061 kb on + strand, within BPHYT_RS36280at 66.061 kb on + strand, within BPHYT_RS36280at 66.062 kb on - strand, within BPHYT_RS36280at 66.062 kb on - strand, within BPHYT_RS36280at 66.062 kb on - strand, within BPHYT_RS36280at 66.063 kb on + strand, within BPHYT_RS36280at 66.079 kb on + strand, within BPHYT_RS36280at 66.080 kb on - strand, within BPHYT_RS36280at 66.110 kb on - strand, within BPHYT_RS36280at 66.259 kb on - strand, within BPHYT_RS36280at 66.259 kb on - strand, within BPHYT_RS36280at 66.323 kb on - strand, within BPHYT_RS36280at 66.323 kb on - strand, within BPHYT_RS36280at 66.323 kb on - strand, within BPHYT_RS36280at 66.323 kb on - strand, within BPHYT_RS36280at 66.323 kb on - strand, within BPHYT_RS36280at 66.366 kb on + strand, within BPHYT_RS36280at 66.367 kb on - strand, within BPHYT_RS36280at 66.469 kb on - strand, within BPHYT_RS36280at 66.484 kb on - strand, within BPHYT_RS36280at 66.484 kb on - strand, within BPHYT_RS36280at 66.484 kb on - strand, within BPHYT_RS36280at 66.484 kb on - strand, within BPHYT_RS36280at 66.484 kb on - strand, within BPHYT_RS36280at 66.487 kb on + strand, within BPHYT_RS36280at 66.487 kb on + strand, within BPHYT_RS36280at 66.488 kb on - strand, within BPHYT_RS36280at 66.488 kb on - strandat 66.488 kb on - strand, within BPHYT_RS36280at 66.530 kb on + strand, within BPHYT_RS36280at 66.530 kb on + strand, within BPHYT_RS36280at 66.531 kb on - strand, within BPHYT_RS36280at 66.588 kb on - strand, within BPHYT_RS36280at 66.588 kb on - strand, within BPHYT_RS36280at 66.695 kb on - strand, within BPHYT_RS36280at 66.695 kb on - strand, within BPHYT_RS36280at 66.829 kb on - strandat 66.829 kb on - strandat 66.887 kb on - strandat 66.939 kb on + strandat 66.940 kb on - strandat 66.940 kb on - strandat 66.958 kb on - strandat 66.958 kb on - strandat 66.961 kb on - strandat 67.091 kb on + strandat 67.479 kb on + strandat 67.842 kb on + strandat 67.842 kb on + strandat 67.842 kb on + strandat 67.842 kb on + strandat 67.842 kb on + strandat 67.842 kb on + strandat 67.842 kb on + strandat 67.842 kb on + strandat 67.843 kb on - strandat 67.843 kb on - strandat 67.843 kb on - strandat 67.843 kb on - strandat 67.843 kb on - strandat 67.843 kb on - strandat 67.843 kb on - strandat 67.843 kb on - strandat 67.843 kb on - strandat 67.845 kb on - strandat 67.862 kb on + strandat 67.862 kb on + strandat 67.863 kb on - strandat 67.868 kb on + strandat 67.869 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1
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64,588 + +2.3
64,823 - +3.3
64,931 - BPHYT_RS36275 0.31 +0.7
65,107 - BPHYT_RS36275 0.77 -0.1
65,107 - BPHYT_RS36275 0.77 +0.7
65,196 + +2.7
65,224 + +0.9
65,224 + -1.5
65,224 + +1.3
65,224 + -0.5
65,224 + -1.3
65,225 - +0.7
65,294 + +2.3
65,366 + BPHYT_RS36280 0.10 +0.7
65,367 - BPHYT_RS36280 0.10 +0.7
65,419 - BPHYT_RS36280 0.13 -0.1
65,458 + +2.3
65,532 + BPHYT_RS36280 0.20 +0.4
65,532 + BPHYT_RS36280 0.20 +0.5
65,532 + BPHYT_RS36280 0.20 -1.3
65,641 - BPHYT_RS36280 0.26 -1.1
65,802 - BPHYT_RS36280 0.36 -0.4
65,891 + BPHYT_RS36280 0.41 +1.9
65,972 + +2.3
66,006 + BPHYT_RS36280 0.48 +2.3
66,036 + BPHYT_RS36280 0.50 -1.7
66,061 + BPHYT_RS36280 0.51 -0.6
66,061 + BPHYT_RS36280 0.51 -2.0
66,062 - BPHYT_RS36280 0.51 -0.8
66,062 - BPHYT_RS36280 0.51 -0.0
66,062 - BPHYT_RS36280 0.51 -0.2
66,063 + BPHYT_RS36280 0.51 +0.7
66,079 + BPHYT_RS36280 0.52 -2.1
66,080 - BPHYT_RS36280 0.52 -0.1
66,110 - BPHYT_RS36280 0.54 -1.0
66,259 - BPHYT_RS36280 0.63 -0.9
66,259 - BPHYT_RS36280 0.63 +0.0
66,323 - BPHYT_RS36280 0.67 -0.2
66,323 - BPHYT_RS36280 0.67 -1.9
66,323 - BPHYT_RS36280 0.67 +0.3
66,323 - BPHYT_RS36280 0.67 -0.9
66,323 - BPHYT_RS36280 0.67 +0.1
66,366 + BPHYT_RS36280 0.69 +0.3
66,367 - BPHYT_RS36280 0.69 -1.7
66,469 - BPHYT_RS36280 0.75 -0.6
66,484 - BPHYT_RS36280 0.76 -1.1
66,484 - BPHYT_RS36280 0.76 +0.8
66,484 - BPHYT_RS36280 0.76 -0.9
66,484 - BPHYT_RS36280 0.76 +0.7
66,484 - BPHYT_RS36280 0.76 -1.0
66,487 + BPHYT_RS36280 0.76 +1.3
66,487 + BPHYT_RS36280 0.76 -0.3
66,488 - BPHYT_RS36280 0.76 -0.6
66,488 - +1.9
66,488 - BPHYT_RS36280 0.76 -0.2
66,530 + BPHYT_RS36280 0.79 +2.3
66,530 + BPHYT_RS36280 0.79 +0.7
66,531 - BPHYT_RS36280 0.79 -0.0
66,588 - BPHYT_RS36280 0.82 -1.1
66,588 - BPHYT_RS36280 0.82 +1.4
66,695 - BPHYT_RS36280 0.89 +0.0
66,695 - BPHYT_RS36280 0.89 -0.3
66,829 - -1.1
66,829 - +2.0
66,887 - +0.1
66,939 + +0.5
66,940 - -1.9
66,940 - -0.5
66,958 - -0.2
66,958 - +0.0
66,961 - +0.7
67,091 + -0.5
67,479 + -0.5
67,842 + +0.2
67,842 + -2.8
67,842 + +1.7
67,842 + -0.2
67,842 + -0.3
67,842 + +0.0
67,842 + +0.0
67,842 + +0.3
67,843 - -1.2
67,843 - +0.7
67,843 - -0.7
67,843 - +0.3
67,843 - -0.3
67,843 - +0.7
67,843 - -1.0
67,843 - +0.1
67,843 - -0.4
67,845 - +1.5
67,862 + +1.2
67,862 + +0.5
67,863 - -0.4
67,868 + +0.4
67,869 - -0.9

Or see this region's nucleotide sequence