Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS35725 and BPHYT_RS35730 are separated by 12 nucleotides BPHYT_RS35730 and BPHYT_RS35735 are separated by 153 nucleotides BPHYT_RS35735 and BPHYT_RS35740 are separated by 330 nucleotides
BPHYT_RS35725: BPHYT_RS35725 - hypothetical protein, at 3,605,467 to 3,606,312
_RS35725
BPHYT_RS35730: BPHYT_RS35730 - hypothetical protein, at 3,606,325 to 3,606,804
_RS35730
BPHYT_RS35735: BPHYT_RS35735 - nitroreductase, at 3,606,958 to 3,607,569
_RS35735
BPHYT_RS35740: BPHYT_RS35740 - MFS transporter, at 3,607,900 to 3,609,147
_RS35740
Position (kb)
3606
3607
3608 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3605.977 kb on - strand, within BPHYT_RS35725 at 3606.046 kb on - strand, within BPHYT_RS35725 at 3606.131 kb on + strand, within BPHYT_RS35725 at 3606.312 kb on + strand at 3606.313 kb on - strand at 3606.313 kb on - strand at 3606.313 kb on - strand at 3606.313 kb on - strand at 3606.313 kb on - strand at 3606.335 kb on + strand at 3606.335 kb on + strand at 3606.335 kb on + strand at 3606.335 kb on + strand at 3606.336 kb on - strand at 3606.336 kb on - strand at 3606.569 kb on + strand, within BPHYT_RS35730 at 3606.569 kb on + strand, within BPHYT_RS35730 at 3606.929 kb on - strand at 3606.930 kb on + strand at 3606.931 kb on - strand at 3606.931 kb on - strand at 3606.931 kb on - strand at 3606.931 kb on - strand at 3607.118 kb on + strand, within BPHYT_RS35735 at 3607.151 kb on + strand, within BPHYT_RS35735 at 3607.517 kb on - strand at 3607.517 kb on - strand at 3607.517 kb on - strand at 3607.517 kb on - strand at 3607.517 kb on - strand at 3607.645 kb on + strand at 3607.645 kb on + strand at 3607.656 kb on + strand at 3607.951 kb on - strand at 3608.137 kb on + strand, within BPHYT_RS35740 at 3608.138 kb on - strand, within BPHYT_RS35740 at 3608.397 kb on + strand, within BPHYT_RS35740 at 3608.470 kb on + strand, within BPHYT_RS35740
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 3,605,977 - BPHYT_RS35725 0.60 -0.4 3,606,046 - BPHYT_RS35725 0.68 -1.5 3,606,131 + BPHYT_RS35725 0.78 -0.5 3,606,312 + -0.9 3,606,313 - -0.2 3,606,313 - -0.9 3,606,313 - +0.6 3,606,313 - -0.0 3,606,313 - +0.1 3,606,335 + -0.0 3,606,335 + +0.7 3,606,335 + -0.4 3,606,335 + +0.1 3,606,336 - +0.2 3,606,336 - +0.1 3,606,569 + BPHYT_RS35730 0.51 +0.1 3,606,569 + BPHYT_RS35730 0.51 +0.5 3,606,929 - +0.1 3,606,930 + -0.0 3,606,931 - +0.6 3,606,931 - +0.2 3,606,931 - +0.2 3,606,931 - +0.1 3,607,118 + BPHYT_RS35735 0.26 -1.1 3,607,151 + BPHYT_RS35735 0.32 -0.8 3,607,517 - +0.1 3,607,517 - +1.0 3,607,517 - -0.3 3,607,517 - +1.9 3,607,517 - +1.6 3,607,645 + +1.9 3,607,645 + -1.3 3,607,656 + -2.0 3,607,951 - -0.1 3,608,137 + BPHYT_RS35740 0.19 +1.0 3,608,138 - BPHYT_RS35740 0.19 -0.7 3,608,397 + BPHYT_RS35740 0.40 -0.6 3,608,470 + BPHYT_RS35740 0.46 +1.2
Or see this region's nucleotide sequence