Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS35715 and BPHYT_RS35720 are separated by 87 nucleotides BPHYT_RS35720 and BPHYT_RS35725 are separated by 20 nucleotides BPHYT_RS35725 and BPHYT_RS35730 are separated by 12 nucleotides BPHYT_RS35730 and BPHYT_RS35735 are separated by 153 nucleotides
BPHYT_RS35715: BPHYT_RS35715 - hypothetical protein, at 3,604,187 to 3,604,663
_RS35715
BPHYT_RS35720: BPHYT_RS35720 - phosphopantetheinyl transferase, at 3,604,751 to 3,605,446
_RS35720
BPHYT_RS35725: BPHYT_RS35725 - hypothetical protein, at 3,605,467 to 3,606,312
_RS35725
BPHYT_RS35730: BPHYT_RS35730 - hypothetical protein, at 3,606,325 to 3,606,804
_RS35730
BPHYT_RS35735: BPHYT_RS35735 - nitroreductase, at 3,606,958 to 3,607,569
_RS35735
Position (kb)
3605
3606
3607 Strain fitness (log2 ratio)
-2
-1
0
1 at 3604.718 kb on + strand at 3604.755 kb on - strand at 3604.857 kb on + strand, within BPHYT_RS35720 at 3604.858 kb on - strand, within BPHYT_RS35720 at 3604.858 kb on - strand, within BPHYT_RS35720 at 3604.858 kb on - strand, within BPHYT_RS35720 at 3604.858 kb on - strand, within BPHYT_RS35720 at 3605.055 kb on + strand, within BPHYT_RS35720 at 3605.055 kb on + strand, within BPHYT_RS35720 at 3605.419 kb on - strand at 3605.419 kb on - strand at 3605.977 kb on - strand, within BPHYT_RS35725 at 3606.046 kb on - strand, within BPHYT_RS35725 at 3606.131 kb on + strand, within BPHYT_RS35725 at 3606.312 kb on + strand at 3606.313 kb on - strand at 3606.313 kb on - strand at 3606.313 kb on - strand at 3606.313 kb on - strand at 3606.313 kb on - strand at 3606.335 kb on + strand at 3606.335 kb on + strand at 3606.335 kb on + strand at 3606.335 kb on + strand at 3606.336 kb on - strand at 3606.336 kb on - strand at 3606.569 kb on + strand, within BPHYT_RS35730 at 3606.569 kb on + strand, within BPHYT_RS35730 at 3606.929 kb on - strand at 3606.930 kb on + strand at 3606.931 kb on - strand at 3606.931 kb on - strand at 3606.931 kb on - strand at 3606.931 kb on - strand at 3607.118 kb on + strand, within BPHYT_RS35735 at 3607.151 kb on + strand, within BPHYT_RS35735
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 3,604,718 + -0.2 3,604,755 - -0.4 3,604,857 + BPHYT_RS35720 0.15 -0.1 3,604,858 - BPHYT_RS35720 0.15 -0.8 3,604,858 - BPHYT_RS35720 0.15 -0.5 3,604,858 - BPHYT_RS35720 0.15 -1.5 3,604,858 - BPHYT_RS35720 0.15 -1.2 3,605,055 + BPHYT_RS35720 0.44 +0.3 3,605,055 + BPHYT_RS35720 0.44 -1.3 3,605,419 - -1.8 3,605,419 - +1.1 3,605,977 - BPHYT_RS35725 0.60 -0.4 3,606,046 - BPHYT_RS35725 0.68 -1.5 3,606,131 + BPHYT_RS35725 0.78 -0.5 3,606,312 + -0.9 3,606,313 - -0.2 3,606,313 - -0.9 3,606,313 - +0.6 3,606,313 - -0.0 3,606,313 - +0.1 3,606,335 + -0.0 3,606,335 + +0.7 3,606,335 + -0.4 3,606,335 + +0.1 3,606,336 - +0.2 3,606,336 - +0.1 3,606,569 + BPHYT_RS35730 0.51 +0.1 3,606,569 + BPHYT_RS35730 0.51 +0.5 3,606,929 - +0.1 3,606,930 + -0.0 3,606,931 - +0.6 3,606,931 - +0.2 3,606,931 - +0.2 3,606,931 - +0.1 3,607,118 + BPHYT_RS35735 0.26 -1.1 3,607,151 + BPHYT_RS35735 0.32 -0.8
Or see this region's nucleotide sequence