Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS34005 and BPHYT_RS34010 are separated by 612 nucleotides BPHYT_RS34010 and BPHYT_RS34015 are separated by 25 nucleotides BPHYT_RS34015 and BPHYT_RS34020 overlap by 1 nucleotides BPHYT_RS34020 and BPHYT_RS34025 overlap by 4 nucleotides
BPHYT_RS34005: BPHYT_RS34005 - hemagglutinin, at 3,221,291 to 3,229,789
_RS34005
BPHYT_RS34010: BPHYT_RS34010 - pilus assembly protein, at 3,230,402 to 3,230,683
_RS34010
BPHYT_RS34015: BPHYT_RS34015 - hypothetical protein, at 3,230,709 to 3,232,235
_RS34015
BPHYT_RS34020: BPHYT_RS34020 - hypothetical protein, at 3,232,235 to 3,233,119
_RS34020
BPHYT_RS34025: BPHYT_RS34025 - hypothetical protein, at 3,233,116 to 3,233,835
_RS34025
Position (kb)
3230
3231
3232
3233 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3229.777 kb on - strand at 3230.102 kb on + strand at 3230.263 kb on - strand at 3230.268 kb on + strand at 3230.459 kb on + strand, within BPHYT_RS34010 at 3230.459 kb on + strand, within BPHYT_RS34010 at 3230.459 kb on + strand, within BPHYT_RS34010 at 3230.459 kb on + strand, within BPHYT_RS34010 at 3230.459 kb on + strand, within BPHYT_RS34010 at 3230.459 kb on + strand, within BPHYT_RS34010 at 3230.602 kb on + strand, within BPHYT_RS34010 at 3230.655 kb on - strand at 3230.655 kb on - strand at 3230.655 kb on - strand at 3230.656 kb on + strand at 3230.885 kb on + strand, within BPHYT_RS34015 at 3230.914 kb on - strand, within BPHYT_RS34015 at 3231.016 kb on - strand, within BPHYT_RS34015 at 3231.022 kb on + strand, within BPHYT_RS34015 at 3231.070 kb on + strand, within BPHYT_RS34015 at 3231.337 kb on - strand, within BPHYT_RS34015 at 3231.506 kb on + strand, within BPHYT_RS34015 at 3231.507 kb on - strand, within BPHYT_RS34015 at 3231.507 kb on - strand, within BPHYT_RS34015 at 3231.507 kb on - strand, within BPHYT_RS34015 at 3231.675 kb on + strand, within BPHYT_RS34015 at 3231.676 kb on - strand, within BPHYT_RS34015 at 3231.677 kb on + strand, within BPHYT_RS34015 at 3231.720 kb on + strand, within BPHYT_RS34015 at 3231.853 kb on - strand, within BPHYT_RS34015 at 3231.921 kb on - strand, within BPHYT_RS34015 at 3232.249 kb on + strand at 3232.249 kb on + strand at 3232.484 kb on + strand, within BPHYT_RS34020 at 3232.485 kb on - strand, within BPHYT_RS34020 at 3232.507 kb on - strand, within BPHYT_RS34020 at 3232.507 kb on - strand, within BPHYT_RS34020 at 3232.721 kb on - strand, within BPHYT_RS34020 at 3232.721 kb on - strand, within BPHYT_RS34020 at 3232.934 kb on - strand, within BPHYT_RS34020 at 3233.025 kb on - strand, within BPHYT_RS34020 at 3233.091 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 3,229,777 - -0.9 3,230,102 + -0.4 3,230,263 - +3.0 3,230,268 + +0.7 3,230,459 + BPHYT_RS34010 0.20 +1.2 3,230,459 + BPHYT_RS34010 0.20 -0.6 3,230,459 + BPHYT_RS34010 0.20 +0.0 3,230,459 + BPHYT_RS34010 0.20 -0.7 3,230,459 + BPHYT_RS34010 0.20 -0.9 3,230,459 + BPHYT_RS34010 0.20 -0.7 3,230,602 + BPHYT_RS34010 0.71 -0.5 3,230,655 - -0.5 3,230,655 - -0.1 3,230,655 - +0.4 3,230,656 + -0.4 3,230,885 + BPHYT_RS34015 0.12 -2.0 3,230,914 - BPHYT_RS34015 0.13 +0.2 3,231,016 - BPHYT_RS34015 0.20 -0.6 3,231,022 + BPHYT_RS34015 0.20 -0.8 3,231,070 + BPHYT_RS34015 0.24 +1.5 3,231,337 - BPHYT_RS34015 0.41 +2.5 3,231,506 + BPHYT_RS34015 0.52 -0.6 3,231,507 - BPHYT_RS34015 0.52 -0.7 3,231,507 - BPHYT_RS34015 0.52 +0.8 3,231,507 - BPHYT_RS34015 0.52 -0.5 3,231,675 + BPHYT_RS34015 0.63 -1.2 3,231,676 - BPHYT_RS34015 0.63 -0.3 3,231,677 + BPHYT_RS34015 0.63 -2.1 3,231,720 + BPHYT_RS34015 0.66 -0.9 3,231,853 - BPHYT_RS34015 0.75 -1.8 3,231,921 - BPHYT_RS34015 0.79 -0.5 3,232,249 + +0.5 3,232,249 + -1.0 3,232,484 + BPHYT_RS34020 0.28 -2.1 3,232,485 - BPHYT_RS34020 0.28 +1.5 3,232,507 - BPHYT_RS34020 0.31 +0.7 3,232,507 - BPHYT_RS34020 0.31 +0.3 3,232,721 - BPHYT_RS34020 0.55 -0.7 3,232,721 - BPHYT_RS34020 0.55 -1.1 3,232,934 - BPHYT_RS34020 0.79 +0.3 3,233,025 - BPHYT_RS34020 0.89 -0.5 3,233,091 + +0.7
Or see this region's nucleotide sequence