Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS33455 and BPHYT_RS33460 are separated by 3 nucleotides BPHYT_RS33460 and BPHYT_RS33465 are separated by 147 nucleotides BPHYT_RS33465 and BPHYT_RS33470 are separated by 154 nucleotides BPHYT_RS33470 and BPHYT_RS33475 are separated by 465 nucleotides
BPHYT_RS33455: BPHYT_RS33455 - protocatechuate 3,4-dioxygenase subunit beta, at 3,092,938 to 3,093,642
_RS33455
BPHYT_RS33460: BPHYT_RS33460 - protocatechuate 3,4-dioxygenase subunit alpha, at 3,093,646 to 3,094,233
_RS33460
BPHYT_RS33465: BPHYT_RS33465 - D-alanyl-D-alanine carboxypeptidase, at 3,094,381 to 3,095,613
_RS33465
BPHYT_RS33470: BPHYT_RS33470 - DNA-binding protein, at 3,095,768 to 3,096,031
_RS33470
BPHYT_RS33475: BPHYT_RS33475 - threonine transporter, at 3,096,497 to 3,097,132
_RS33475
Position (kb)
3094
3095
3096 Strain fitness (log2 ratio)
-2
-1
0
1 at 3093.412 kb on - strand, within BPHYT_RS33455 at 3093.589 kb on + strand at 3093.688 kb on + strand at 3094.066 kb on + strand, within BPHYT_RS33460 at 3094.255 kb on + strand at 3094.269 kb on - strand at 3094.288 kb on + strand at 3094.570 kb on + strand, within BPHYT_RS33465 at 3094.765 kb on - strand, within BPHYT_RS33465 at 3094.875 kb on + strand, within BPHYT_RS33465 at 3094.876 kb on - strand, within BPHYT_RS33465 at 3095.159 kb on - strand, within BPHYT_RS33465 at 3095.269 kb on - strand, within BPHYT_RS33465 at 3095.770 kb on - strand at 3095.852 kb on - strand, within BPHYT_RS33470 at 3095.852 kb on - strand, within BPHYT_RS33470 at 3095.852 kb on - strand, within BPHYT_RS33470 at 3095.852 kb on - strand, within BPHYT_RS33470 at 3095.852 kb on - strand, within BPHYT_RS33470 at 3095.852 kb on - strand at 3095.852 kb on - strand, within BPHYT_RS33470 at 3095.854 kb on + strand, within BPHYT_RS33470 at 3095.854 kb on + strand, within BPHYT_RS33470 at 3095.872 kb on - strand, within BPHYT_RS33470 at 3095.932 kb on + strand, within BPHYT_RS33470 at 3096.036 kb on + strand at 3096.036 kb on + strand at 3096.037 kb on - strand at 3096.054 kb on + strand at 3096.054 kb on + strand at 3096.071 kb on - strand at 3096.103 kb on + strand at 3096.124 kb on - strand at 3096.124 kb on - strand at 3096.276 kb on - strand at 3096.291 kb on - strand at 3096.392 kb on - strand at 3096.453 kb on + strand at 3096.453 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 3,093,412 - BPHYT_RS33455 0.67 -0.9 3,093,589 + +0.7 3,093,688 + +0.1 3,094,066 + BPHYT_RS33460 0.71 -0.3 3,094,255 + +0.8 3,094,269 - -1.3 3,094,288 + +0.9 3,094,570 + BPHYT_RS33465 0.15 -1.1 3,094,765 - BPHYT_RS33465 0.31 -1.5 3,094,875 + BPHYT_RS33465 0.40 +1.1 3,094,876 - BPHYT_RS33465 0.40 +0.7 3,095,159 - BPHYT_RS33465 0.63 -0.4 3,095,269 - BPHYT_RS33465 0.72 -1.0 3,095,770 - -0.1 3,095,852 - BPHYT_RS33470 0.32 -1.0 3,095,852 - BPHYT_RS33470 0.32 -1.1 3,095,852 - BPHYT_RS33470 0.32 -0.1 3,095,852 - BPHYT_RS33470 0.32 -0.8 3,095,852 - BPHYT_RS33470 0.32 -1.2 3,095,852 - +1.0 3,095,852 - BPHYT_RS33470 0.32 -0.9 3,095,854 + BPHYT_RS33470 0.33 -1.3 3,095,854 + BPHYT_RS33470 0.33 -1.3 3,095,872 - BPHYT_RS33470 0.39 -0.3 3,095,932 + BPHYT_RS33470 0.62 +0.2 3,096,036 + +0.3 3,096,036 + -0.2 3,096,037 - -0.4 3,096,054 + -2.6 3,096,054 + -0.6 3,096,071 - +0.4 3,096,103 + -0.7 3,096,124 - -0.1 3,096,124 - +0.4 3,096,276 - -0.4 3,096,291 - -0.1 3,096,392 - +0.6 3,096,453 + -1.0 3,096,453 + -0.1
Or see this region's nucleotide sequence