Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS33355 and BPHYT_RS33360 are separated by 24 nucleotides BPHYT_RS33360 and BPHYT_RS33365 are separated by 105 nucleotides BPHYT_RS33365 and BPHYT_RS33370 are separated by 80 nucleotides
BPHYT_RS33355: BPHYT_RS33355 - oxalyl-CoA decarboxylase, at 3,066,064 to 3,067,806
_RS33355
BPHYT_RS33360: BPHYT_RS33360 - formyl-CoA transferase, at 3,067,831 to 3,069,081
_RS33360
BPHYT_RS33365: BPHYT_RS33365 - PAS sensor protein, at 3,069,187 to 3,069,618
_RS33365
BPHYT_RS33370: BPHYT_RS33370 - LysR family transcriptional regulator, at 3,069,699 to 3,070,625
_RS33370
Position (kb) 3067
3068
3069
3070 Strain fitness (log2 ratio) -2
-1
0
1 at 3067.538 kb on + strand, within BPHYT_RS33355 at 3067.539 kb on - strand, within BPHYT_RS33355 at 3068.228 kb on - strand, within BPHYT_RS33360 at 3068.519 kb on - strand, within BPHYT_RS33360 at 3068.630 kb on - strand, within BPHYT_RS33360 at 3069.089 kb on + strand at 3069.327 kb on + strand, within BPHYT_RS33365 at 3069.450 kb on + strand, within BPHYT_RS33365 at 3069.892 kb on - strand, within BPHYT_RS33370
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 3,067,538 + BPHYT_RS33355 0.85 +0.1 3,067,539 - BPHYT_RS33355 0.85 -0.9 3,068,228 - BPHYT_RS33360 0.32 +1.1 3,068,519 - BPHYT_RS33360 0.55 -1.8 3,068,630 - BPHYT_RS33360 0.64 -2.0 3,069,089 + +1.0 3,069,327 + BPHYT_RS33365 0.32 -2.1 3,069,450 + BPHYT_RS33365 0.61 +0.2 3,069,892 - BPHYT_RS33370 0.21 -0.7
Or see this region's nucleotide sequence