Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS31955 and BPHYT_RS31960 are separated by 206 nucleotides BPHYT_RS31960 and BPHYT_RS31965 are separated by 98 nucleotides
BPHYT_RS31955: BPHYT_RS31955 - 3-hydroxyisobutyrate dehydrogenase, at 2,729,015 to 2,729,917
_RS31955
BPHYT_RS31960: BPHYT_RS31960 - hypothetical protein, at 2,730,124 to 2,731,071
_RS31960
BPHYT_RS31965: BPHYT_RS31965 - mandelate racemase, at 2,731,170 to 2,732,303
_RS31965
Position (kb)
2730
2731
2732 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2729.165 kb on - strand, within BPHYT_RS31955 at 2729.165 kb on - strand, within BPHYT_RS31955 at 2729.174 kb on - strand, within BPHYT_RS31955 at 2729.243 kb on - strand, within BPHYT_RS31955 at 2729.255 kb on - strand, within BPHYT_RS31955 at 2729.298 kb on - strand, within BPHYT_RS31955 at 2729.414 kb on + strand, within BPHYT_RS31955 at 2729.415 kb on - strand, within BPHYT_RS31955 at 2729.441 kb on + strand, within BPHYT_RS31955 at 2729.442 kb on - strand, within BPHYT_RS31955 at 2729.442 kb on - strand, within BPHYT_RS31955 at 2729.442 kb on - strand, within BPHYT_RS31955 at 2729.455 kb on - strand, within BPHYT_RS31955 at 2729.455 kb on - strand, within BPHYT_RS31955 at 2729.510 kb on - strand, within BPHYT_RS31955 at 2729.735 kb on + strand, within BPHYT_RS31955 at 2729.827 kb on - strand at 2730.222 kb on + strand, within BPHYT_RS31960 at 2730.667 kb on + strand, within BPHYT_RS31960 at 2730.758 kb on + strand, within BPHYT_RS31960 at 2730.759 kb on - strand, within BPHYT_RS31960 at 2731.081 kb on + strand at 2731.120 kb on + strand at 2731.145 kb on + strand at 2731.145 kb on + strand at 2731.277 kb on - strand at 2731.494 kb on - strand, within BPHYT_RS31965 at 2731.657 kb on - strand, within BPHYT_RS31965 at 2731.760 kb on + strand, within BPHYT_RS31965 at 2731.764 kb on - strand, within BPHYT_RS31965 at 2731.764 kb on - strand, within BPHYT_RS31965 at 2731.771 kb on - strand, within BPHYT_RS31965 at 2731.777 kb on + strand, within BPHYT_RS31965 at 2731.796 kb on - strand, within BPHYT_RS31965 at 2731.945 kb on + strand, within BPHYT_RS31965 at 2732.038 kb on + strand, within BPHYT_RS31965
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 2,729,165 - BPHYT_RS31955 0.17 -1.4 2,729,165 - BPHYT_RS31955 0.17 +0.1 2,729,174 - BPHYT_RS31955 0.18 -0.6 2,729,243 - BPHYT_RS31955 0.25 -0.5 2,729,255 - BPHYT_RS31955 0.27 +0.9 2,729,298 - BPHYT_RS31955 0.31 -1.1 2,729,414 + BPHYT_RS31955 0.44 +0.5 2,729,415 - BPHYT_RS31955 0.44 +0.4 2,729,441 + BPHYT_RS31955 0.47 -0.2 2,729,442 - BPHYT_RS31955 0.47 +0.5 2,729,442 - BPHYT_RS31955 0.47 -0.7 2,729,442 - BPHYT_RS31955 0.47 -2.1 2,729,455 - BPHYT_RS31955 0.49 +0.3 2,729,455 - BPHYT_RS31955 0.49 -0.6 2,729,510 - BPHYT_RS31955 0.55 -0.0 2,729,735 + BPHYT_RS31955 0.80 -0.1 2,729,827 - +0.0 2,730,222 + BPHYT_RS31960 0.10 -0.3 2,730,667 + BPHYT_RS31960 0.57 +3.1 2,730,758 + BPHYT_RS31960 0.67 +0.2 2,730,759 - BPHYT_RS31960 0.67 -1.5 2,731,081 + +1.2 2,731,120 + -0.1 2,731,145 + +0.5 2,731,145 + +2.2 2,731,277 - -2.0 2,731,494 - BPHYT_RS31965 0.29 -0.3 2,731,657 - BPHYT_RS31965 0.43 +0.5 2,731,760 + BPHYT_RS31965 0.52 -0.9 2,731,764 - BPHYT_RS31965 0.52 -1.3 2,731,764 - BPHYT_RS31965 0.52 +0.2 2,731,771 - BPHYT_RS31965 0.53 -0.6 2,731,777 + BPHYT_RS31965 0.54 -1.5 2,731,796 - BPHYT_RS31965 0.55 +0.1 2,731,945 + BPHYT_RS31965 0.68 -0.1 2,732,038 + BPHYT_RS31965 0.77 -1.7
Or see this region's nucleotide sequence