Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS31485 and BPHYT_RS31490 are separated by 188 nucleotides BPHYT_RS31490 and BPHYT_RS31495 overlap by 7 nucleotides BPHYT_RS31495 and BPHYT_RS31500 are separated by 31 nucleotides BPHYT_RS31500 and BPHYT_RS31505 are separated by 18 nucleotides
BPHYT_RS31485: BPHYT_RS31485 - hypothetical protein, at 2,618,562 to 2,619,296
_RS31485
BPHYT_RS31490: BPHYT_RS31490 - hypothetical protein, at 2,619,485 to 2,619,700
_RS31490
BPHYT_RS31495: BPHYT_RS31495 - transposase, at 2,619,694 to 2,620,014
_RS31495
BPHYT_RS31500: BPHYT_RS31500 - hypothetical protein, at 2,620,046 to 2,620,249
_RS31500
BPHYT_RS31505: BPHYT_RS31505 - hypothetical protein, at 2,620,268 to 2,621,038
_RS31505
Position (kb)
2620
2621
2622 Strain fitness (log2 ratio)
-1
0
1 at 2619.681 kb on - strand at 2619.830 kb on + strand, within BPHYT_RS31495 at 2619.882 kb on + strand, within BPHYT_RS31495 at 2619.882 kb on + strand, within BPHYT_RS31495 at 2619.986 kb on + strand at 2620.059 kb on + strand at 2620.060 kb on - strand at 2620.315 kb on + strand at 2620.530 kb on + strand, within BPHYT_RS31505 at 2620.530 kb on + strand, within BPHYT_RS31505 at 2620.531 kb on - strand, within BPHYT_RS31505 at 2620.830 kb on + strand, within BPHYT_RS31505 at 2620.962 kb on - strand at 2620.962 kb on - strand at 2621.062 kb on + strand at 2621.351 kb on - strand at 2621.351 kb on - strand at 2621.462 kb on + strand at 2621.510 kb on - strand at 2621.545 kb on + strand at 2621.788 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 2,619,681 - +0.0 2,619,830 + BPHYT_RS31495 0.42 -0.8 2,619,882 + BPHYT_RS31495 0.59 -0.6 2,619,882 + BPHYT_RS31495 0.59 -1.1 2,619,986 + +0.7 2,620,059 + +0.3 2,620,060 - +1.3 2,620,315 + -1.7 2,620,530 + BPHYT_RS31505 0.34 -0.3 2,620,530 + BPHYT_RS31505 0.34 -1.0 2,620,531 - BPHYT_RS31505 0.34 +1.5 2,620,830 + BPHYT_RS31505 0.73 +0.8 2,620,962 - +0.2 2,620,962 - -0.1 2,621,062 + +0.4 2,621,351 - +0.2 2,621,351 - +0.7 2,621,462 + -0.4 2,621,510 - -0.3 2,621,545 + -0.1 2,621,788 - -0.1
Or see this region's nucleotide sequence