Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS30375 and BPHYT_RS30380 overlap by 11 nucleotides BPHYT_RS30380 and BPHYT_RS30385 are separated by 48 nucleotides BPHYT_RS30385 and BPHYT_RS30390 are separated by 41 nucleotides BPHYT_RS30390 and BPHYT_RS30395 are separated by 141 nucleotides
BPHYT_RS30375: BPHYT_RS30375 - hypothetical protein, at 2,370,616 to 2,372,166
_RS30375
BPHYT_RS30380: BPHYT_RS30380 - glyoxalase, at 2,372,156 to 2,373,106
_RS30380
BPHYT_RS30385: BPHYT_RS30385 - hypothetical protein, at 2,373,155 to 2,373,406
_RS30385
BPHYT_RS30390: BPHYT_RS30390 - phosphohydrolase, at 2,373,448 to 2,374,089
_RS30390
BPHYT_RS30395: BPHYT_RS30395 - small mechanosensitive ion channel protein MscS, at 2,374,231 to 2,375,700
_RS30395
Position (kb)
2373
2374 Strain fitness (log2 ratio)
-2
-1
0
1 at 2372.520 kb on + strand, within BPHYT_RS30380 at 2372.520 kb on + strand, within BPHYT_RS30380 at 2372.521 kb on - strand, within BPHYT_RS30380 at 2372.799 kb on + strand, within BPHYT_RS30380 at 2372.799 kb on + strand, within BPHYT_RS30380 at 2372.800 kb on - strand, within BPHYT_RS30380 at 2372.920 kb on - strand, within BPHYT_RS30380 at 2373.113 kb on - strand at 2373.422 kb on - strand at 2373.500 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 2,372,520 + BPHYT_RS30380 0.38 -2.1 2,372,520 + BPHYT_RS30380 0.38 +0.7 2,372,521 - BPHYT_RS30380 0.38 +0.0 2,372,799 + BPHYT_RS30380 0.68 -2.6 2,372,799 + BPHYT_RS30380 0.68 -1.4 2,372,800 - BPHYT_RS30380 0.68 -2.0 2,372,920 - BPHYT_RS30380 0.80 +0.6 2,373,113 - -0.2 2,373,422 - -1.4 2,373,500 + -0.4
Or see this region's nucleotide sequence