Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS28755

Experiment: 36hr PHYTO4 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS28745 and BPHYT_RS28750 are separated by 65 nucleotidesBPHYT_RS28750 and BPHYT_RS28755 are separated by 86 nucleotidesBPHYT_RS28755 and BPHYT_RS28760 are separated by 326 nucleotides BPHYT_RS28745: BPHYT_RS28745 - 2-keto-4-pentenoate hydratase, at 2,002,340 to 2,003,149 _RS28745 BPHYT_RS28750: BPHYT_RS28750 - IclR family transcriptional regulator, at 2,003,215 to 2,003,499 _RS28750 BPHYT_RS28755: BPHYT_RS28755 - 3-(2-hydroxyphenyl) propionic acid transporter, at 2,003,586 to 2,005,019 _RS28755 BPHYT_RS28760: BPHYT_RS28760 - porin, at 2,005,346 to 2,006,581 _RS28760 Position (kb) 2003 2004 2005 2006Strain fitness (log2 ratio) -2 -1 0 1at 2002.778 kb on - strand, within BPHYT_RS28745at 2002.782 kb on - strand, within BPHYT_RS28745at 2002.843 kb on + strand, within BPHYT_RS28745at 2003.426 kb on - strand, within BPHYT_RS28750at 2003.549 kb on + strandat 2003.549 kb on + strandat 2003.549 kb on + strandat 2003.549 kb on + strandat 2003.550 kb on - strandat 2003.553 kb on + strandat 2004.876 kb on + strandat 2005.255 kb on + strandat 2005.728 kb on - strand, within BPHYT_RS28760

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1
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2,002,778 - BPHYT_RS28745 0.54 +0.3
2,002,782 - BPHYT_RS28745 0.55 +0.6
2,002,843 + BPHYT_RS28745 0.62 +0.3
2,003,426 - BPHYT_RS28750 0.74 -2.3
2,003,549 + -2.1
2,003,549 + +0.0
2,003,549 + -0.7
2,003,549 + -0.2
2,003,550 - +1.5
2,003,553 + +1.2
2,004,876 + +0.2
2,005,255 + -0.5
2,005,728 - BPHYT_RS28760 0.31 +0.5

Or see this region's nucleotide sequence