Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS27785

Experiment: 36hr PHYTO4 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS27780 and BPHYT_RS27785 are separated by 102 nucleotidesBPHYT_RS27785 and BPHYT_RS27790 are separated by 85 nucleotides BPHYT_RS27780: BPHYT_RS27780 - acetyl-CoA synthetase, at 1,792,688 to 1,794,664 _RS27780 BPHYT_RS27785: BPHYT_RS27785 - hypothetical protein, at 1,794,767 to 1,795,372 _RS27785 BPHYT_RS27790: BPHYT_RS27790 - poly-beta-hydroxybutyrate polymerase, at 1,795,458 to 1,797,314 _RS27790 Position (kb) 1794 1795 1796Strain fitness (log2 ratio) -1 0 1at 1795.253 kb on + strand, within BPHYT_RS27785at 1795.359 kb on + strandat 1795.446 kb on + strandat 1795.447 kb on - strandat 1795.657 kb on + strand, within BPHYT_RS27790at 1795.657 kb on + strand, within BPHYT_RS27790at 1795.856 kb on - strand, within BPHYT_RS27790at 1796.069 kb on - strand, within BPHYT_RS27790

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1
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1,795,253 + BPHYT_RS27785 0.80 +0.4
1,795,359 + -0.2
1,795,446 + +0.2
1,795,447 - +0.3
1,795,657 + BPHYT_RS27790 0.11 -0.1
1,795,657 + BPHYT_RS27790 0.11 +0.5
1,795,856 - BPHYT_RS27790 0.21 +0.3
1,796,069 - BPHYT_RS27790 0.33 -0.9

Or see this region's nucleotide sequence