Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS27125

Experiment: 36hr PHYTO4 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS27115 and BPHYT_RS27120 are separated by 222 nucleotidesBPHYT_RS27120 and BPHYT_RS27125 are separated by 72 nucleotidesBPHYT_RS27125 and BPHYT_RS27130 are separated by 830 nucleotides BPHYT_RS27115: BPHYT_RS27115 - peptidase M20, at 1,660,597 to 1,662,057 _RS27115 BPHYT_RS27120: BPHYT_RS27120 - hypothetical protein, at 1,662,280 to 1,662,591 _RS27120 BPHYT_RS27125: BPHYT_RS27125 - hypothetical protein, at 1,662,664 to 1,662,936 _RS27125 BPHYT_RS27130: BPHYT_RS27130 - heat-labile enterotoxin A chain, at 1,663,767 to 1,666,664 _RS27130 Position (kb) 1662 1663Strain fitness (log2 ratio) -1 0 1at 1661.894 kb on + strand, within BPHYT_RS27115at 1662.100 kb on + strandat 1662.446 kb on - strand, within BPHYT_RS27120at 1662.830 kb on + strand, within BPHYT_RS27125at 1663.128 kb on - strandat 1663.252 kb on + strandat 1663.271 kb on + strandat 1663.307 kb on - strandat 1663.493 kb on + strandat 1663.514 kb on - strandat 1663.514 kb on - strandat 1663.632 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1
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1,661,894 + BPHYT_RS27115 0.89 +0.7
1,662,100 + +0.1
1,662,446 - BPHYT_RS27120 0.53 +0.1
1,662,830 + BPHYT_RS27125 0.61 -1.2
1,663,128 - +1.7
1,663,252 + -0.3
1,663,271 + -0.2
1,663,307 - +0.6
1,663,493 + +0.8
1,663,514 - -0.2
1,663,514 - +0.0
1,663,632 + -0.3

Or see this region's nucleotide sequence