Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS26655 and BPHYT_RS26660 are separated by 105 nucleotides BPHYT_RS26660 and BPHYT_RS26665 are separated by 62 nucleotides BPHYT_RS26665 and BPHYT_RS26670 are separated by 2 nucleotides
BPHYT_RS26655: BPHYT_RS26655 - 5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase, at 1,547,735 to 1,550,029
_RS26655
BPHYT_RS26660: BPHYT_RS26660 - precorrin-4 C11-methyltransferase, at 1,550,135 to 1,550,860
_RS26660
BPHYT_RS26665: BPHYT_RS26665 - cobalt-precorrin-6X reductase, at 1,550,923 to 1,551,645
_RS26665
BPHYT_RS26670: BPHYT_RS26670 - Cobalt-precorrin-5B C(1)-methyltransferase, at 1,551,648 to 1,552,745
_RS26670
Position (kb)
1550
1551 Strain fitness (log2 ratio)
-2
-1
0
1 at 1549.170 kb on + strand, within BPHYT_RS26655 at 1549.651 kb on + strand, within BPHYT_RS26655 at 1549.657 kb on + strand, within BPHYT_RS26655 at 1549.788 kb on - strand, within BPHYT_RS26655 at 1550.325 kb on + strand, within BPHYT_RS26660 at 1550.336 kb on + strand, within BPHYT_RS26660 at 1550.480 kb on - strand, within BPHYT_RS26660 at 1550.846 kb on - strand at 1551.085 kb on + strand, within BPHYT_RS26665 at 1551.200 kb on - strand, within BPHYT_RS26665 at 1551.230 kb on + strand, within BPHYT_RS26665 at 1551.257 kb on - strand, within BPHYT_RS26665 at 1551.479 kb on + strand, within BPHYT_RS26665 at 1551.489 kb on + strand, within BPHYT_RS26665 at 1551.567 kb on + strand, within BPHYT_RS26665 at 1551.568 kb on - strand, within BPHYT_RS26665 at 1551.569 kb on + strand, within BPHYT_RS26665 at 1551.570 kb on - strand, within BPHYT_RS26665
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 1,549,170 + BPHYT_RS26655 0.63 -1.0 1,549,651 + BPHYT_RS26655 0.83 -0.5 1,549,657 + BPHYT_RS26655 0.84 -0.6 1,549,788 - BPHYT_RS26655 0.89 -0.7 1,550,325 + BPHYT_RS26660 0.26 -0.6 1,550,336 + BPHYT_RS26660 0.28 -1.2 1,550,480 - BPHYT_RS26660 0.48 -0.2 1,550,846 - +0.4 1,551,085 + BPHYT_RS26665 0.22 -0.6 1,551,200 - BPHYT_RS26665 0.38 -1.4 1,551,230 + BPHYT_RS26665 0.42 -0.6 1,551,257 - BPHYT_RS26665 0.46 +1.0 1,551,479 + BPHYT_RS26665 0.77 -2.7 1,551,489 + BPHYT_RS26665 0.78 +0.2 1,551,567 + BPHYT_RS26665 0.89 +0.2 1,551,568 - BPHYT_RS26665 0.89 +1.6 1,551,569 + BPHYT_RS26665 0.89 +0.4 1,551,570 - BPHYT_RS26665 0.89 +0.8
Or see this region's nucleotide sequence