Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS25700 and BPHYT_RS25705 are separated by 20 nucleotides BPHYT_RS25705 and BPHYT_RS25710 are separated by 231 nucleotides BPHYT_RS25710 and BPHYT_RS25715 are separated by 153 nucleotides
BPHYT_RS25700: BPHYT_RS25700 - MFS transporter, at 1,338,296 to 1,339,696
_RS25700
BPHYT_RS25705: BPHYT_RS25705 - aldehyde dehydrogenase, at 1,339,717 to 1,341,228
_RS25705
BPHYT_RS25710: BPHYT_RS25710 - hypothetical protein, at 1,341,460 to 1,341,675
_RS25710
BPHYT_RS25715: BPHYT_RS25715 - transposase, at 1,341,829 to 1,343,262
_RS25715
Position (kb) 1339
1340
1341
1342 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1338.860 kb on + strand, within BPHYT_RS25700 at 1339.108 kb on + strand, within BPHYT_RS25700 at 1339.406 kb on + strand, within BPHYT_RS25700 at 1339.407 kb on - strand, within BPHYT_RS25700 at 1339.716 kb on + strand at 1339.717 kb on - strand at 1340.075 kb on - strand, within BPHYT_RS25705 at 1340.244 kb on + strand, within BPHYT_RS25705 at 1340.691 kb on - strand, within BPHYT_RS25705 at 1341.105 kb on - strand at 1341.274 kb on + strand at 1341.275 kb on - strand at 1341.404 kb on - strand at 1341.412 kb on + strand at 1341.616 kb on + strand, within BPHYT_RS25710 at 1341.679 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 1,338,860 + BPHYT_RS25700 0.40 -0.6 1,339,108 + BPHYT_RS25700 0.58 +0.1 1,339,406 + BPHYT_RS25700 0.79 -2.2 1,339,407 - BPHYT_RS25700 0.79 -1.3 1,339,716 + -1.1 1,339,717 - -1.7 1,340,075 - BPHYT_RS25705 0.24 +0.2 1,340,244 + BPHYT_RS25705 0.35 -0.3 1,340,691 - BPHYT_RS25705 0.64 -1.4 1,341,105 - +0.5 1,341,274 + -0.6 1,341,275 - +3.0 1,341,404 - +3.0 1,341,412 + -1.0 1,341,616 + BPHYT_RS25710 0.72 -0.7 1,341,679 + +0.2
Or see this region's nucleotide sequence