Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS25690 and BPHYT_RS25695 are separated by 56 nucleotides BPHYT_RS25695 and BPHYT_RS25700 are separated by 127 nucleotides BPHYT_RS25700 and BPHYT_RS25705 are separated by 20 nucleotides
BPHYT_RS25690: BPHYT_RS25690 - GntR family transcriptional regulator, at 1,336,571 to 1,337,380
_RS25690
BPHYT_RS25695: BPHYT_RS25695 - enoyl-CoA hydratase, at 1,337,437 to 1,338,168
_RS25695
BPHYT_RS25700: BPHYT_RS25700 - MFS transporter, at 1,338,296 to 1,339,696
_RS25700
BPHYT_RS25705: BPHYT_RS25705 - aldehyde dehydrogenase, at 1,339,717 to 1,341,228
_RS25705
Position (kb) 1338
1339
1340 Strain fitness (log2 ratio) -2
-1
0
1 at 1337.422 kb on - strand at 1338.404 kb on + strand at 1338.554 kb on - strand, within BPHYT_RS25700 at 1338.558 kb on - strand, within BPHYT_RS25700 at 1338.860 kb on + strand, within BPHYT_RS25700 at 1339.108 kb on + strand, within BPHYT_RS25700 at 1339.406 kb on + strand, within BPHYT_RS25700 at 1339.407 kb on - strand, within BPHYT_RS25700 at 1339.716 kb on + strand at 1339.717 kb on - strand at 1340.075 kb on - strand, within BPHYT_RS25705 at 1340.244 kb on + strand, within BPHYT_RS25705 at 1340.691 kb on - strand, within BPHYT_RS25705
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 1,337,422 - -0.4 1,338,404 + -1.1 1,338,554 - BPHYT_RS25700 0.18 +0.6 1,338,558 - BPHYT_RS25700 0.19 -0.9 1,338,860 + BPHYT_RS25700 0.40 -0.6 1,339,108 + BPHYT_RS25700 0.58 +0.1 1,339,406 + BPHYT_RS25700 0.79 -2.2 1,339,407 - BPHYT_RS25700 0.79 -1.3 1,339,716 + -1.1 1,339,717 - -1.7 1,340,075 - BPHYT_RS25705 0.24 +0.2 1,340,244 + BPHYT_RS25705 0.35 -0.3 1,340,691 - BPHYT_RS25705 0.64 -1.4
Or see this region's nucleotide sequence