Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS25685

Experiment: 36hr PHYTO4 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS25675 and BPHYT_RS25680 are separated by 78 nucleotidesBPHYT_RS25680 and BPHYT_RS25685 are separated by 233 nucleotidesBPHYT_RS25685 and BPHYT_RS25690 are separated by 14 nucleotidesBPHYT_RS25690 and BPHYT_RS25695 are separated by 56 nucleotides BPHYT_RS25675: BPHYT_RS25675 - MFS transporter, at 1,333,684 to 1,335,096 _RS25675 BPHYT_RS25680: BPHYT_RS25680 - GntR family transcriptional regulator, at 1,335,175 to 1,335,804 _RS25680 BPHYT_RS25685: BPHYT_RS25685 - damage-inducible protein DinB, at 1,336,038 to 1,336,556 _RS25685 BPHYT_RS25690: BPHYT_RS25690 - GntR family transcriptional regulator, at 1,336,571 to 1,337,380 _RS25690 BPHYT_RS25695: BPHYT_RS25695 - enoyl-CoA hydratase, at 1,337,437 to 1,338,168 _RS25695 Position (kb) 1336 1337Strain fitness (log2 ratio) -3 -2 -1 0 1at 1335.426 kb on + strand, within BPHYT_RS25680at 1335.915 kb on + strandat 1335.916 kb on - strandat 1336.148 kb on - strand, within BPHYT_RS25685at 1336.335 kb on + strandat 1336.403 kb on - strand, within BPHYT_RS25685at 1336.403 kb on - strand, within BPHYT_RS25685at 1336.403 kb on - strand, within BPHYT_RS25685at 1336.406 kb on + strand, within BPHYT_RS25685at 1337.192 kb on + strand, within BPHYT_RS25690at 1337.422 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1
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1,335,426 + BPHYT_RS25680 0.40 +0.6
1,335,915 + +0.4
1,335,916 - -0.7
1,336,148 - BPHYT_RS25685 0.21 -0.3
1,336,335 + -1.1
1,336,403 - BPHYT_RS25685 0.70 -0.2
1,336,403 - BPHYT_RS25685 0.70 -1.0
1,336,403 - BPHYT_RS25685 0.70 -0.7
1,336,406 + BPHYT_RS25685 0.71 -3.0
1,337,192 + BPHYT_RS25690 0.77 +0.1
1,337,422 - -0.4

Or see this region's nucleotide sequence