Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS24365 and BPHYT_RS24370 are separated by 303 nucleotides BPHYT_RS24370 and BPHYT_RS24375 are separated by 73 nucleotides BPHYT_RS24375 and BPHYT_RS24380 overlap by 4 nucleotides
BPHYT_RS24365: BPHYT_RS24365 - ornithine cyclodeaminase, at 1,031,424 to 1,032,359
_RS24365
BPHYT_RS24370: BPHYT_RS24370 - glucose-6-phosphate 1-dehydrogenase, at 1,032,663 to 1,034,144
_RS24370
BPHYT_RS24375: BPHYT_RS24375 - type VI secretion system protein ImpK, at 1,034,218 to 1,035,000
_RS24375
BPHYT_RS24380: BPHYT_RS24380 - type VI secretion protein, at 1,034,997 to 1,036,343
_RS24380
Position (kb)
1032
1033
1034
1035 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1032.118 kb on + strand, within BPHYT_RS24365 at 1032.118 kb on + strand, within BPHYT_RS24365 at 1032.287 kb on + strand at 1032.418 kb on + strand at 1032.428 kb on + strand at 1032.429 kb on - strand at 1032.473 kb on + strand at 1032.474 kb on - strand at 1032.491 kb on + strand at 1032.491 kb on + strand at 1032.766 kb on - strand at 1032.846 kb on + strand, within BPHYT_RS24370 at 1032.847 kb on - strand, within BPHYT_RS24370 at 1033.209 kb on + strand, within BPHYT_RS24370 at 1033.210 kb on - strand, within BPHYT_RS24370 at 1033.353 kb on + strand, within BPHYT_RS24370 at 1033.353 kb on + strand, within BPHYT_RS24370 at 1033.354 kb on - strand, within BPHYT_RS24370 at 1033.359 kb on - strand at 1033.359 kb on - strand, within BPHYT_RS24370 at 1033.643 kb on + strand, within BPHYT_RS24370 at 1033.863 kb on - strand, within BPHYT_RS24370 at 1033.927 kb on - strand, within BPHYT_RS24370 at 1034.063 kb on + strand at 1034.142 kb on + strand at 1034.142 kb on + strand at 1034.277 kb on - strand at 1034.312 kb on + strand, within BPHYT_RS24375 at 1034.316 kb on + strand, within BPHYT_RS24375 at 1034.317 kb on - strand, within BPHYT_RS24375 at 1034.331 kb on - strand, within BPHYT_RS24375 at 1034.753 kb on + strand, within BPHYT_RS24375 at 1034.753 kb on + strand, within BPHYT_RS24375 at 1034.925 kb on - strand at 1034.993 kb on + strand at 1034.994 kb on - strand at 1035.052 kb on + strand at 1035.053 kb on - strand at 1035.053 kb on - strand at 1035.081 kb on - strand at 1035.081 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 1,032,118 + BPHYT_RS24365 0.74 +1.2 1,032,118 + BPHYT_RS24365 0.74 -1.0 1,032,287 + +0.3 1,032,418 + -1.3 1,032,428 + -0.1 1,032,429 - -0.3 1,032,473 + -0.6 1,032,474 - +0.3 1,032,491 + +0.6 1,032,491 + +0.2 1,032,766 - -0.8 1,032,846 + BPHYT_RS24370 0.12 +0.6 1,032,847 - BPHYT_RS24370 0.12 -0.4 1,033,209 + BPHYT_RS24370 0.37 +0.2 1,033,210 - BPHYT_RS24370 0.37 +1.5 1,033,353 + BPHYT_RS24370 0.47 +1.0 1,033,353 + BPHYT_RS24370 0.47 +0.1 1,033,354 - BPHYT_RS24370 0.47 +0.8 1,033,359 - +0.8 1,033,359 - BPHYT_RS24370 0.47 +1.1 1,033,643 + BPHYT_RS24370 0.66 -0.6 1,033,863 - BPHYT_RS24370 0.81 -2.3 1,033,927 - BPHYT_RS24370 0.85 -0.6 1,034,063 + -0.1 1,034,142 + -1.3 1,034,142 + +0.2 1,034,277 - -0.1 1,034,312 + BPHYT_RS24375 0.12 -0.2 1,034,316 + BPHYT_RS24375 0.13 +0.1 1,034,317 - BPHYT_RS24375 0.13 -0.6 1,034,331 - BPHYT_RS24375 0.14 +0.3 1,034,753 + BPHYT_RS24375 0.68 +0.3 1,034,753 + BPHYT_RS24375 0.68 -1.5 1,034,925 - -0.7 1,034,993 + -0.6 1,034,994 - +0.1 1,035,052 + -1.1 1,035,053 - -1.1 1,035,053 - +1.2 1,035,081 - +2.4 1,035,081 - +0.2
Or see this region's nucleotide sequence