Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS24280 and BPHYT_RS24285 are separated by 134 nucleotides BPHYT_RS24285 and BPHYT_RS24290 are separated by 92 nucleotides BPHYT_RS24290 and BPHYT_RS24295 are separated by 476 nucleotides
BPHYT_RS24280: BPHYT_RS24280 - DSBA oxidoreductase, at 1,013,303 to 1,014,844
_RS24280
BPHYT_RS24285: BPHYT_RS24285 - TetR family transcriptional regulator, at 1,014,979 to 1,015,704
_RS24285
BPHYT_RS24290: BPHYT_RS24290 - hypothetical protein, at 1,015,797 to 1,016,516
_RS24290
BPHYT_RS24295: BPHYT_RS24295 - response regulator receiver, at 1,016,993 to 1,017,505
_RS24295
Position (kb) 1015
1016
1017 Strain fitness (log2 ratio) -2
-1
0
1 at 1015.037 kb on + strand at 1015.222 kb on + strand, within BPHYT_RS24285 at 1015.223 kb on - strand, within BPHYT_RS24285 at 1015.764 kb on - strand at 1015.858 kb on + strand at 1015.858 kb on + strand at 1016.423 kb on + strand, within BPHYT_RS24290 at 1016.423 kb on + strand, within BPHYT_RS24290 at 1016.424 kb on - strand, within BPHYT_RS24290 at 1016.424 kb on - strand, within BPHYT_RS24290 at 1016.424 kb on - strand, within BPHYT_RS24290 at 1016.445 kb on + strand at 1016.445 kb on + strand at 1016.446 kb on - strand at 1016.704 kb on - strand at 1016.704 kb on - strand at 1016.706 kb on + strand at 1016.706 kb on + strand at 1016.707 kb on - strand at 1016.707 kb on - strand at 1016.943 kb on + strand at 1017.302 kb on + strand, within BPHYT_RS24295
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 1,015,037 + -0.6 1,015,222 + BPHYT_RS24285 0.33 -1.9 1,015,223 - BPHYT_RS24285 0.34 +0.4 1,015,764 - +1.2 1,015,858 + +0.4 1,015,858 + +0.5 1,016,423 + BPHYT_RS24290 0.87 -0.7 1,016,423 + BPHYT_RS24290 0.87 -0.6 1,016,424 - BPHYT_RS24290 0.87 -0.1 1,016,424 - BPHYT_RS24290 0.87 -0.1 1,016,424 - BPHYT_RS24290 0.87 +0.9 1,016,445 + +0.0 1,016,445 + +0.7 1,016,446 - -0.5 1,016,704 - +0.4 1,016,704 - -0.6 1,016,706 + +0.2 1,016,706 + +0.0 1,016,707 - -2.4 1,016,707 - +0.0 1,016,943 + -0.2 1,017,302 + BPHYT_RS24295 0.60 -0.8
Or see this region's nucleotide sequence