Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS24275 and BPHYT_RS24280 are separated by 398 nucleotides BPHYT_RS24280 and BPHYT_RS24285 are separated by 134 nucleotides BPHYT_RS24285 and BPHYT_RS24290 are separated by 92 nucleotides
BPHYT_RS24275: BPHYT_RS24275 - alpha-ketoglutarate decarboxylase, at 1,011,630 to 1,012,904
_RS24275
BPHYT_RS24280: BPHYT_RS24280 - DSBA oxidoreductase, at 1,013,303 to 1,014,844
_RS24280
BPHYT_RS24285: BPHYT_RS24285 - TetR family transcriptional regulator, at 1,014,979 to 1,015,704
_RS24285
BPHYT_RS24290: BPHYT_RS24290 - hypothetical protein, at 1,015,797 to 1,016,516
_RS24290
Position (kb) 1013
1014
1015 Strain fitness (log2 ratio) -2
-1
0
1 at 1012.379 kb on - strand, within BPHYT_RS24275 at 1012.529 kb on - strand, within BPHYT_RS24275 at 1012.814 kb on - strand at 1013.088 kb on + strand at 1013.700 kb on + strand, within BPHYT_RS24280 at 1013.701 kb on - strand, within BPHYT_RS24280 at 1013.701 kb on - strand, within BPHYT_RS24280 at 1014.186 kb on + strand, within BPHYT_RS24280 at 1014.186 kb on + strand, within BPHYT_RS24280 at 1014.253 kb on + strand, within BPHYT_RS24280 at 1014.253 kb on + strand, within BPHYT_RS24280 at 1014.643 kb on + strand, within BPHYT_RS24280 at 1015.037 kb on + strand at 1015.222 kb on + strand, within BPHYT_RS24285 at 1015.223 kb on - strand, within BPHYT_RS24285 at 1015.764 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 1,012,379 - BPHYT_RS24275 0.59 -0.2 1,012,529 - BPHYT_RS24275 0.71 -0.3 1,012,814 - +0.4 1,013,088 + -0.2 1,013,700 + BPHYT_RS24280 0.26 -0.1 1,013,701 - BPHYT_RS24280 0.26 +1.5 1,013,701 - BPHYT_RS24280 0.26 +0.2 1,014,186 + BPHYT_RS24280 0.57 -0.7 1,014,186 + BPHYT_RS24280 0.57 -0.6 1,014,253 + BPHYT_RS24280 0.62 -0.1 1,014,253 + BPHYT_RS24280 0.62 +0.2 1,014,643 + BPHYT_RS24280 0.87 +1.0 1,015,037 + -0.6 1,015,222 + BPHYT_RS24285 0.33 -1.9 1,015,223 - BPHYT_RS24285 0.34 +0.4 1,015,764 - +1.2
Or see this region's nucleotide sequence