Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS24040 and BPHYT_RS24045 are separated by 21 nucleotides BPHYT_RS24045 and BPHYT_RS24050 are separated by 88 nucleotides BPHYT_RS24050 and BPHYT_RS24055 are separated by 306 nucleotides
BPHYT_RS24040: BPHYT_RS24040 - amino acid ABC transporter, at 958,084 to 958,746
_RS24040
BPHYT_RS24045: BPHYT_RS24045 - amino acid ABC transporter, at 958,768 to 959,418
_RS24045
BPHYT_RS24050: BPHYT_RS24050 - amino acid ABC transporter, at 959,507 to 960,334
_RS24050
BPHYT_RS24055: BPHYT_RS24055 - oxidoreductase, at 960,641 to 961,630
_RS24055
Position (kb) 959
960
961 Strain fitness (log2 ratio) -2
-1
0
1 at 958.521 kb on - strand, within BPHYT_RS24040 at 958.571 kb on + strand, within BPHYT_RS24040 at 958.835 kb on + strand, within BPHYT_RS24045 at 959.195 kb on + strand, within BPHYT_RS24045 at 959.195 kb on + strand, within BPHYT_RS24045 at 959.434 kb on + strand at 959.511 kb on + strand at 959.673 kb on + strand, within BPHYT_RS24050 at 959.994 kb on + strand, within BPHYT_RS24050 at 959.995 kb on - strand, within BPHYT_RS24050 at 960.426 kb on - strand at 960.426 kb on - strand at 960.908 kb on + strand, within BPHYT_RS24055 at 961.093 kb on - strand, within BPHYT_RS24055 at 961.312 kb on + strand, within BPHYT_RS24055
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 958,521 - BPHYT_RS24040 0.66 +0.6 958,571 + BPHYT_RS24040 0.73 -0.2 958,835 + BPHYT_RS24045 0.10 +1.4 959,195 + BPHYT_RS24045 0.66 -0.4 959,195 + BPHYT_RS24045 0.66 +0.9 959,434 + -0.2 959,511 + +0.1 959,673 + BPHYT_RS24050 0.20 -1.7 959,994 + BPHYT_RS24050 0.59 +0.7 959,995 - BPHYT_RS24050 0.59 -0.2 960,426 - -0.6 960,426 - -2.6 960,908 + BPHYT_RS24055 0.27 +0.1 961,093 - BPHYT_RS24055 0.46 +0.3 961,312 + BPHYT_RS24055 0.68 +0.2
Or see this region's nucleotide sequence