Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23900 and BPHYT_RS23905 are separated by 30 nucleotides BPHYT_RS23905 and BPHYT_RS23910 overlap by 1 nucleotides BPHYT_RS23910 and BPHYT_RS23915 overlap by 1 nucleotides
BPHYT_RS23900: BPHYT_RS23900 - LysR family transcriptional regulator, at 928,800 to 929,738
_RS23900
BPHYT_RS23905: BPHYT_RS23905 - malonate decarboxylase subunit epsilon, at 929,769 to 930,701
_RS23905
BPHYT_RS23910: BPHYT_RS23910 - phosphoribosyl-dephospho-CoA transferase, at 930,701 to 931,399
_RS23910
BPHYT_RS23915: BPHYT_RS23915 - malonate decarboxylase subunit gamma, at 931,399 to 932,217
_RS23915
Position (kb) 929
930
931 Strain fitness (log2 ratio) -2
-1
0
1
2 at 929.105 kb on + strand, within BPHYT_RS23900 at 929.106 kb on - strand, within BPHYT_RS23900 at 929.559 kb on + strand, within BPHYT_RS23900 at 929.559 kb on + strand, within BPHYT_RS23900 at 929.628 kb on + strand, within BPHYT_RS23900 at 929.629 kb on - strand, within BPHYT_RS23900 at 929.698 kb on - strand at 930.007 kb on + strand, within BPHYT_RS23905 at 930.038 kb on - strand, within BPHYT_RS23905 at 930.038 kb on - strand, within BPHYT_RS23905 at 930.576 kb on - strand, within BPHYT_RS23905 at 931.139 kb on + strand, within BPHYT_RS23910
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 929,105 + BPHYT_RS23900 0.32 -0.8 929,106 - BPHYT_RS23900 0.33 +1.1 929,559 + BPHYT_RS23900 0.81 -2.5 929,559 + BPHYT_RS23900 0.81 +0.1 929,628 + BPHYT_RS23900 0.88 +0.1 929,629 - BPHYT_RS23900 0.88 +0.6 929,698 - +0.8 930,007 + BPHYT_RS23905 0.26 -1.6 930,038 - BPHYT_RS23905 0.29 -0.2 930,038 - BPHYT_RS23905 0.29 -1.0 930,576 - BPHYT_RS23905 0.86 -1.0 931,139 + BPHYT_RS23910 0.63 +2.1
Or see this region's nucleotide sequence