Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22665 and BPHYT_RS22670 are separated by 157 nucleotides BPHYT_RS22670 and BPHYT_RS22675 are separated by 190 nucleotides BPHYT_RS22675 and BPHYT_RS22680 are separated by 318 nucleotides BPHYT_RS22680 and BPHYT_RS22685 overlap by 4 nucleotides
BPHYT_RS22665: BPHYT_RS22665 - hypothetical protein, at 641,360 to 641,620
_RS22665
BPHYT_RS22670: BPHYT_RS22670 - hypothetical protein, at 641,778 to 642,110
_RS22670
BPHYT_RS22675: BPHYT_RS22675 - 4-hydroxyphenylpyruvate dioxygenase, at 642,301 to 644,184
_RS22675
BPHYT_RS22680: BPHYT_RS22680 - 3-dehydroquinate dehydratase, at 644,503 to 644,958
_RS22680
BPHYT_RS22685: BPHYT_RS22685 - shikimate 5-dehydrogenase, at 644,955 to 645,869
_RS22685
Position (kb) 642
643
644
645 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 641.313 kb on + strand at 641.362 kb on - strand at 641.683 kb on + strand at 641.683 kb on + strand at 641.683 kb on + strand at 641.684 kb on - strand at 641.761 kb on + strand at 641.851 kb on + strand, within BPHYT_RS22670 at 642.110 kb on + strand at 642.111 kb on - strand at 642.203 kb on + strand at 642.423 kb on - strand at 642.462 kb on - strand at 642.472 kb on - strand at 642.533 kb on + strand, within BPHYT_RS22675 at 642.534 kb on - strand, within BPHYT_RS22675 at 642.536 kb on + strand, within BPHYT_RS22675 at 642.644 kb on + strand, within BPHYT_RS22675 at 642.645 kb on - strand, within BPHYT_RS22675 at 642.645 kb on - strand, within BPHYT_RS22675 at 642.645 kb on - strand, within BPHYT_RS22675 at 642.645 kb on - strand, within BPHYT_RS22675 at 642.648 kb on + strand, within BPHYT_RS22675 at 642.648 kb on + strand, within BPHYT_RS22675 at 642.648 kb on + strand, within BPHYT_RS22675 at 642.649 kb on - strand, within BPHYT_RS22675 at 642.753 kb on - strand, within BPHYT_RS22675 at 642.793 kb on + strand, within BPHYT_RS22675 at 643.491 kb on - strand, within BPHYT_RS22675 at 644.575 kb on + strand, within BPHYT_RS22680 at 644.575 kb on + strand, within BPHYT_RS22680 at 644.748 kb on + strand, within BPHYT_RS22680 at 644.809 kb on - strand, within BPHYT_RS22680 at 644.851 kb on + strand, within BPHYT_RS22680 at 644.902 kb on + strand, within BPHYT_RS22680 at 644.903 kb on - strand, within BPHYT_RS22680 at 644.903 kb on - strand, within BPHYT_RS22680 at 644.903 kb on - strand, within BPHYT_RS22680 at 644.906 kb on + strand, within BPHYT_RS22680 at 644.907 kb on - strand, within BPHYT_RS22680 at 644.938 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 641,313 + +0.7 641,362 - -0.4 641,683 + -1.0 641,683 + -1.7 641,683 + +1.7 641,684 - -0.5 641,761 + -0.7 641,851 + BPHYT_RS22670 0.22 +1.4 642,110 + -1.0 642,111 - -1.0 642,203 + -1.6 642,423 - -1.6 642,462 - -0.6 642,472 - +0.0 642,533 + BPHYT_RS22675 0.12 +0.2 642,534 - BPHYT_RS22675 0.12 -0.6 642,536 + BPHYT_RS22675 0.12 +1.9 642,644 + BPHYT_RS22675 0.18 +0.8 642,645 - BPHYT_RS22675 0.18 -0.3 642,645 - BPHYT_RS22675 0.18 +0.3 642,645 - BPHYT_RS22675 0.18 -0.5 642,645 - BPHYT_RS22675 0.18 -1.7 642,648 + BPHYT_RS22675 0.18 -0.8 642,648 + BPHYT_RS22675 0.18 -2.2 642,648 + BPHYT_RS22675 0.18 +0.4 642,649 - BPHYT_RS22675 0.18 -0.2 642,753 - BPHYT_RS22675 0.24 +0.8 642,793 + BPHYT_RS22675 0.26 -0.1 643,491 - BPHYT_RS22675 0.63 +3.5 644,575 + BPHYT_RS22680 0.16 +0.5 644,575 + BPHYT_RS22680 0.16 -0.5 644,748 + BPHYT_RS22680 0.54 +0.5 644,809 - BPHYT_RS22680 0.67 +0.2 644,851 + BPHYT_RS22680 0.76 +2.5 644,902 + BPHYT_RS22680 0.88 +0.2 644,903 - BPHYT_RS22680 0.88 +0.7 644,903 - BPHYT_RS22680 0.88 +0.3 644,903 - BPHYT_RS22680 0.88 +0.4 644,906 + BPHYT_RS22680 0.88 -0.2 644,907 - BPHYT_RS22680 0.89 -0.7 644,938 - +0.8
Or see this region's nucleotide sequence