Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS21910

Experiment: 36hr PHYTO4 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS21905 and BPHYT_RS21910 are separated by 75 nucleotidesBPHYT_RS21910 and BPHYT_RS21915 are separated by 17 nucleotidesBPHYT_RS21915 and BPHYT_RS21920 overlap by 4 nucleotides BPHYT_RS21905: BPHYT_RS21905 - IclR family transcriptional regulator, at 473,096 to 473,845 _RS21905 BPHYT_RS21910: BPHYT_RS21910 - ABC transporter permease, at 473,921 to 474,676 _RS21910 BPHYT_RS21915: BPHYT_RS21915 - ABC transporter permease, at 474,694 to 475,362 _RS21915 BPHYT_RS21920: BPHYT_RS21920 - ABC transporter ATP-binding protein, at 475,359 to 476,135 _RS21920 Position (kb) 473 474 475Strain fitness (log2 ratio) -1 0 1 2at 473.697 kb on + strand, within BPHYT_RS21905at 474.212 kb on + strand, within BPHYT_RS21910at 474.213 kb on - strand, within BPHYT_RS21910at 474.485 kb on + strand, within BPHYT_RS21910at 474.738 kb on - strandat 474.989 kb on - strand, within BPHYT_RS21915at 475.388 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1
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473,697 + BPHYT_RS21905 0.80 -1.7
474,212 + BPHYT_RS21910 0.38 +1.1
474,213 - BPHYT_RS21910 0.39 +1.7
474,485 + BPHYT_RS21910 0.75 +0.3
474,738 - -0.1
474,989 - BPHYT_RS21915 0.44 +1.5
475,388 + +2.4

Or see this region's nucleotide sequence