Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21205 and BPHYT_RS21210 are separated by 116 nucleotides BPHYT_RS21210 and BPHYT_RS21215 are separated by 130 nucleotides
BPHYT_RS21205: BPHYT_RS21205 - high-affinity nickel-transporter, at 319,331 to 320,455
_RS21205
BPHYT_RS21210: BPHYT_RS21210 - hypothetical protein, at 320,572 to 321,891
_RS21210
BPHYT_RS21215: BPHYT_RS21215 - Crp/Fnr family transcriptional regulator, at 322,022 to 322,678
_RS21215
Position (kb) 320
321
322 Strain fitness (log2 ratio) -1
0
1
2 at 319.766 kb on - strand, within BPHYT_RS21205 at 319.899 kb on + strand, within BPHYT_RS21205 at 319.900 kb on - strand, within BPHYT_RS21205 at 320.050 kb on - strand, within BPHYT_RS21205 at 320.211 kb on + strand, within BPHYT_RS21205 at 320.211 kb on + strand, within BPHYT_RS21205 at 320.451 kb on + strand at 320.606 kb on + strand at 320.618 kb on + strand at 320.618 kb on + strand at 320.696 kb on + strand at 320.696 kb on + strand at 320.697 kb on - strand at 320.697 kb on - strand at 320.735 kb on + strand, within BPHYT_RS21210 at 320.996 kb on + strand, within BPHYT_RS21210 at 321.208 kb on - strand, within BPHYT_RS21210 at 321.407 kb on - strand, within BPHYT_RS21210 at 321.422 kb on + strand, within BPHYT_RS21210 at 321.422 kb on + strand, within BPHYT_RS21210 at 321.423 kb on - strand, within BPHYT_RS21210 at 321.749 kb on + strand, within BPHYT_RS21210 at 322.023 kb on + strand at 322.024 kb on - strand at 322.324 kb on + strand, within BPHYT_RS21215 at 322.324 kb on + strand, within BPHYT_RS21215 at 322.324 kb on + strand, within BPHYT_RS21215 at 322.324 kb on + strand, within BPHYT_RS21215 at 322.325 kb on - strand, within BPHYT_RS21215 at 322.325 kb on - strand, within BPHYT_RS21215 at 322.545 kb on + strand, within BPHYT_RS21215 at 322.546 kb on - strand, within BPHYT_RS21215 at 322.546 kb on - strand, within BPHYT_RS21215 at 322.575 kb on + strand, within BPHYT_RS21215 at 322.813 kb on + strand at 322.813 kb on + strand at 322.813 kb on + strand at 322.845 kb on + strand at 322.846 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 319,766 - BPHYT_RS21205 0.39 +0.7 319,899 + BPHYT_RS21205 0.50 +0.3 319,900 - BPHYT_RS21205 0.51 +0.2 320,050 - BPHYT_RS21205 0.64 +0.8 320,211 + BPHYT_RS21205 0.78 -0.9 320,211 + BPHYT_RS21205 0.78 -0.1 320,451 + -0.1 320,606 + -0.1 320,618 + +0.2 320,618 + -0.9 320,696 + -0.5 320,696 + +0.4 320,697 - -1.3 320,697 - -0.1 320,735 + BPHYT_RS21210 0.12 -0.9 320,996 + BPHYT_RS21210 0.32 -0.3 321,208 - BPHYT_RS21210 0.48 -0.2 321,407 - BPHYT_RS21210 0.63 +1.0 321,422 + BPHYT_RS21210 0.64 -0.3 321,422 + BPHYT_RS21210 0.64 -1.5 321,423 - BPHYT_RS21210 0.64 -0.6 321,749 + BPHYT_RS21210 0.89 -1.6 322,023 + +0.9 322,024 - +0.8 322,324 + BPHYT_RS21215 0.46 +0.2 322,324 + BPHYT_RS21215 0.46 +1.0 322,324 + BPHYT_RS21215 0.46 +1.2 322,324 + BPHYT_RS21215 0.46 +2.6 322,325 - BPHYT_RS21215 0.46 +1.0 322,325 - BPHYT_RS21215 0.46 +1.9 322,545 + BPHYT_RS21215 0.80 +1.6 322,546 - BPHYT_RS21215 0.80 +0.1 322,546 - BPHYT_RS21215 0.80 -1.4 322,575 + BPHYT_RS21215 0.84 -0.2 322,813 + +1.6 322,813 + +0.2 322,813 + -0.4 322,845 + -0.4 322,846 - +0.7
Or see this region's nucleotide sequence