Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20125 and BPHYT_RS20130 are separated by 826 nucleotides BPHYT_RS20130 and BPHYT_RS20135 are separated by 196 nucleotides BPHYT_RS20135 and BPHYT_RS20140 are separated by 37 nucleotides
BPHYT_RS20125: BPHYT_RS20125 - histidine kinase, at 58,296 to 58,511
_RS20125
BPHYT_RS20130: BPHYT_RS20130 - diguanylate phosphodiesterase, at 59,338 to 60,105
_RS20130
BPHYT_RS20135: BPHYT_RS20135 - UDP-N-acetyl-D-mannosamine transferase, at 60,302 to 61,060
_RS20135
BPHYT_RS20140: BPHYT_RS20140 - hypothetical protein, at 61,098 to 62,264
_RS20140
Position (kb) 59
60
61 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 58.696 kb on - strand at 58.897 kb on + strand at 58.898 kb on - strand at 58.944 kb on - strand at 59.046 kb on - strand at 59.146 kb on - strand at 59.159 kb on + strand at 59.160 kb on - strand at 59.175 kb on + strand at 59.230 kb on + strand at 59.243 kb on - strand at 59.721 kb on + strand, within BPHYT_RS20130 at 59.733 kb on - strand, within BPHYT_RS20130 at 59.748 kb on - strand, within BPHYT_RS20130 at 60.135 kb on - strand at 60.135 kb on - strand at 60.401 kb on - strand, within BPHYT_RS20135 at 60.628 kb on + strand, within BPHYT_RS20135 at 60.855 kb on - strand, within BPHYT_RS20135 at 60.875 kb on + strand, within BPHYT_RS20135 at 60.875 kb on + strand, within BPHYT_RS20135
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 58,696 - +1.1 58,897 + +0.6 58,898 - -0.0 58,944 - +1.7 59,046 - +0.1 59,146 - -0.4 59,159 + +1.0 59,160 - +4.4 59,175 + +0.6 59,230 + -0.4 59,243 - +1.6 59,721 + BPHYT_RS20130 0.50 -0.6 59,733 - BPHYT_RS20130 0.51 -2.8 59,748 - BPHYT_RS20130 0.53 -1.3 60,135 - -1.0 60,135 - +1.6 60,401 - BPHYT_RS20135 0.13 -1.2 60,628 + BPHYT_RS20135 0.43 -2.0 60,855 - BPHYT_RS20135 0.73 +1.6 60,875 + BPHYT_RS20135 0.75 +0.6 60,875 + BPHYT_RS20135 0.75 -0.6
Or see this region's nucleotide sequence