Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS19935 and BPHYT_RS19940 are separated by 5 nucleotides BPHYT_RS19940 and BPHYT_RS19945 are separated by 470 nucleotides BPHYT_RS19945 and BPHYT_RS19950 are separated by 67 nucleotides BPHYT_RS19950 and BPHYT_RS19955 overlap by 4 nucleotides
BPHYT_RS19935: BPHYT_RS19935 - protein serine/threonine phosphatase, at 18,986 to 19,756
_RS19935
BPHYT_RS19940: BPHYT_RS19940 - hypothetical protein, at 19,762 to 20,049
_RS19940
BPHYT_RS19945: BPHYT_RS19945 - hypothetical protein, at 20,520 to 21,017
_RS19945
BPHYT_RS19950: BPHYT_RS19950 - hypothetical protein, at 21,085 to 21,342
_RS19950
BPHYT_RS19955: BPHYT_RS19955 - LysR family transcriptional regulator, at 21,339 to 22,217
_RS19955
Position (kb) 20
21
22 Strain fitness (log2 ratio) -2
-1
0
1 at 19.770 kb on + strand at 19.831 kb on + strand, within BPHYT_RS19940 at 19.832 kb on - strand, within BPHYT_RS19940 at 19.871 kb on + strand, within BPHYT_RS19940 at 19.934 kb on + strand, within BPHYT_RS19940 at 19.998 kb on + strand, within BPHYT_RS19940 at 20.040 kb on + strand at 20.041 kb on - strand at 20.106 kb on + strand at 20.107 kb on - strand at 20.120 kb on - strand at 20.120 kb on - strand at 20.643 kb on + strand, within BPHYT_RS19945 at 20.670 kb on + strand, within BPHYT_RS19945 at 20.778 kb on + strand, within BPHYT_RS19945 at 21.200 kb on - strand, within BPHYT_RS19950 at 21.203 kb on + strand, within BPHYT_RS19950 at 21.203 kb on + strand, within BPHYT_RS19950 at 21.203 kb on + strand, within BPHYT_RS19950 at 21.203 kb on + strand, within BPHYT_RS19950 at 21.204 kb on - strand, within BPHYT_RS19950 at 21.204 kb on - strand, within BPHYT_RS19950 at 21.204 kb on - strand, within BPHYT_RS19950
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 19,770 + -1.0 19,831 + BPHYT_RS19940 0.24 +1.3 19,832 - BPHYT_RS19940 0.24 +0.7 19,871 + BPHYT_RS19940 0.38 +1.6 19,934 + BPHYT_RS19940 0.60 +0.3 19,998 + BPHYT_RS19940 0.82 -1.9 20,040 + +0.0 20,041 - -0.8 20,106 + +0.2 20,107 - +0.0 20,120 - +0.3 20,120 - -1.1 20,643 + BPHYT_RS19945 0.25 -0.6 20,670 + BPHYT_RS19945 0.30 -0.8 20,778 + BPHYT_RS19945 0.52 -0.4 21,200 - BPHYT_RS19950 0.45 -1.0 21,203 + BPHYT_RS19950 0.46 +0.2 21,203 + BPHYT_RS19950 0.46 -1.2 21,203 + BPHYT_RS19950 0.46 +0.9 21,203 + BPHYT_RS19950 0.46 -0.8 21,204 - BPHYT_RS19950 0.46 +0.0 21,204 - BPHYT_RS19950 0.46 +1.2 21,204 - BPHYT_RS19950 0.46 +0.4
Or see this region's nucleotide sequence